Preparation method of parachicken blood phili bacillus liquid culture medium

A technology of liquid culture medium for Haemophilus paragallinarum, which is applied in the field of preparation of liquid culture medium for Haemophilus paragallinarum, which can solve the problems of high cost and low bacterial content

Inactive Publication Date: 2006-01-25
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its disadvantages are: after the cultivation of Haemophilus ...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Take 100 grams of chicken breast muscle, wash and mince, add 800 ml of distilled water, boil for 10 minutes, filter to remove the meat residue, and adjust the volume of chicken broth to 900 ml. Under sterile conditions, take 100 ml of sheep blood, put it in a -40°C refrigerator and freeze and thaw three times to obtain lysed blood. Add 5 grams of peptone, 2 grams of yeast extract, 5 grams of glucose, and 10 grams of sodium chloride into 900 ml of chicken broth, adjust the pH value to 7.4 after dissolving, sterilize in an autoclave at 121 ° C for 20 minutes, and cool Add 100 ml of sterile animal lysed blood to prepare Haemophilus paragallinarum liquid culture medium.

Embodiment 2

[0014] Take 100 grams of chicken breast muscle, wash and mince, add 800 ml of distilled water, boil for 10 minutes, filter to remove the meat residue, and adjust the volume of chicken broth to 900 ml. Under sterile conditions, take 100 ml of chicken blood, freeze and thaw three times in a -40°C refrigerator to obtain lysed blood. Following preparation method is with embodiment 1.

[0015] The Haemophilus paragallinarum liquid medium prepared in the above examples 1 and 2 is suitable for the growth of Haemophilus paragallinarum. In this medium, Haemophilus paragallinarum was cultivated at 37°C for 36 hours, and the thalline amount was up to 10 11 CFU / ml, providing high-quality antigens for the clinical preparation of Haemophilus paragallinarum vaccines.

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Abstract

The invention relates to a fit chicken hemophilus liquid cultivating medium that belongs to bioengineering field. It includes the following steps: washing chicken pectoral muscle and grinding, adding distilled water, boiling, filtering to remove meat residue and adjusting chicken soup capacity; under the condition of asepsis, getting animal blood and freezing to form degradation blood; adding peptone, yeastrel, dextrose, sodium chloride, into chicken soup, after solving, sterilizing in high pressure sterilizer; after cooling, adding degradation blood into the soup, the fit chicken hemophilus liquid cultivating medium would be made. The invention improves the fungus containing quantity of the cultivating medium and lowers cost.

Description

technical field [0001] The invention relates to a method for preparing a biological culture medium, in particular to a method for preparing a Haemophilus paragallinarum liquid culture medium. used in the field of bioengineering. Background technique [0002] At present, the medium used for breeding Haemophilus paragallinarum generally uses solid chocolate medium, and also uses heart and brain soup, chicken embryo or cell culture, but the amount of prepared bacteria is small, and the general bacteria content is 10. 8 CFU / ml, (CFU is colony forming unit) can prepare vaccine after being concentrated, and the cost is high. Haemophilus paragallinarum CVCC258 and CVCC269 are the strains in the "Chinese strain catalog" (English version, published by Machinery Industry Press in October 1992, edited by China Microbial Strain Collection Management Committee), China Veterinary Microbiology Collection Center as the Chinese The organization under the Microbial Cultures Preservation Man...

Claims

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Application Information

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IPC IPC(8): C12N1/20
Inventor 刘永德汪毅叶陈梁吴祖立
Owner SHANGHAI JIAO TONG UNIV
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