Suspension-aid agent for vaccine of coccidiosis
A technology of suspending agent and coccidiosis, which is applied in the direction of medical preparations of non-active ingredients, protozoan antigen components, liquid delivery, etc., to achieve the effect of reducing stress, uniform drinking water of chickens, and ensuring uniformity
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example 1
[0011] Example 1 Static test of oocyst sedimentation
[0012] 1 Materials and methods:
[0013] 1.1 Coccidia oocyst mixture: containing Eimeria tenella, Eimeria tenella, E.necatrix, E.maxima and pile-type Eimeria 4 species of worm (E.acervulina) were mixed at a ratio of 1:1:1:1, and a coccidiosis vaccine solution with 1 million oocysts / ml was formulated with 2.5% potassium dichromate solution.
[0014] 1.2 Test grouping and treatment:
[0015] One group: Shake the coccidia oocyst mixture fully, absorb 2ml and add it to 1000ml drinking water, stir repeatedly from different directions (clockwise, counterclockwise, up and down); weigh 7g of the suspension-improving powder of the present invention, and slowly add In the above-mentioned coccidian oocyst liquid, stir repeatedly from different directions (clockwise, counterclockwise, up and down) while adding, until the suspending agent is completely dissolved, and the coccidia oocyst liquid is paste.
[0016] Grou...
example 2
[0022] Example 2 Comparison of the effects of different immunization methods of chicken coccidiosis attenuated virus vaccine
[0023] 1 Materials and methods:
[0024] 1.1 Test chicks
[0025] 3,000 1-day-old commercial chicklings were purchased from Gaoming Huigang Chicken Farm, raised on the ground with litter, and fed ad libitum.
[0026] 1.2 Chicken coccidiosis attenuated live oocyst quadrivalent vaccine (canonical vaccine - COVAC)
[0027] Developed by our unit, it contains Eimeria tenella, E. necatrix, E. maxima and E. acervulina) 4 species, the immunization dose was 1200 oocysts / chicken.
[0028] 1.3 Feed
[0029] Provided by Guangdong Guanghong Jiujiang Feed Co., Ltd., it is a full-price feed without adding any anticoccidial drugs.
[0030] 1.4 Experimental design
[0031] The above-mentioned 3000 test chicks were randomly divided into 3 groups, 1000 in each group. The first group is the immunization group without drinking water, the second group is the...
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