Method of treating diabetes using plant argyrolobium roseum extract

Technology of an extract, diabetes, application field in a disease or condition

Inactive Publication Date: 2005-11-30
COUNCIL OF SCI & IND RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report that Argyrolobium roseum has any medicinal or biological effects on animals or mammals such as humans
In particular, no description was found of the use of this natural product to prepare hyperglycemic states

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The dosage of streptozotocin is used to induce diabetes similar to NIDDM, most of clinical patients are MDDM diabetes. When the NIDDM diabetic rats were treated with the alcohol extract of the pink silver bean plant for 2 to 3 weeks, the rats returned to a normal state, while the diabetic symptoms of the rats in the NIDDM control group continued to exist and died after a period of time.

Embodiment 2

[0035]1kg of pink silver beans are dried in the shade to make powder. The powdered plants were extracted with ethanol (5 L) for 20 hours. The alcoholic extract was dried under vacuum. The yield was 91 gms. The dried alcoholic extract was macerated sequentially with hexane and chloroform to give 8.2 gms and 39.3 gms of extract, respectively. Using silica gel with a mesh size of 60-120 microns to repeat column chromatography, 230mgsl of flavonoid glycosides were isolated from the chloroform extract. Using normal, fasting for 18h, glucose loaded (glucose loaded) and hyperglycemia rats induced by streptozotocin to detect, it can be seen that the extract (200mg / kg orally) has obvious activity of lowering blood sugar. The onset of effect is within 1 / 2 hour, and the effect lasts for more than 5 hours. In rats fasted for 18 hours, the blood glucose drop of the above dose was recorded as 26 mg / dl (n=24), compared with that of tolbutamide (50 mg / kg, orally, n=9) and Glide Pyrazine ...

Embodiment 3

[0038] 1kg of pink silver beans are dried in the shade to make powder. The powdered plants were soaked 4 times with 95% methanol (3 liters each). The alcoholic extract was vacuum dried to yield 85 gms. The alcoholic extract was macerated sequentially with hexane and acetone to give 6.7 gms and 49.6 gms of extract, respectively. The compound was repeatedly separated from the acetone extract by silica gel column chromatography with a mesh diameter of 60-120 microns to obtain flavonoid glycosides (170 mgs). Oral administration of the extract (200mg / kg / day) for 2 to 3 weeks has significant curative effect on non-insulin-dependent diabetes mellitus, and the curative effect is measured by the following indicators: i) blood sugar level, ii) impact on body weight, iii) animal Survival, iv) water intake, urine excretion & presence of glucose in urine, v) general symptoms of animals, and vi) ant patronage of rats excreted urine.

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Abstract

The present invention relates to a process for isolating plant Argyrobium roseum extract that contains flavonoid glycoside, wherein the extract possesses hypoglycaemic activity. The present invention also contemplates a composition containing the extract and a method of treating various hyperglycaemic conditions including non-insulin dependent diabetes mellitus disease condition by administering the extract.

Description

technical field [0001] The present invention relates to an anti-diabetic extract obtained from Argyrolobium roseum plants, a method for isolating the extract, and an application of the extract in treating diseases or conditions related to hyperglycemia. Background technique [0002] Pink silver bean (Argyrolobium roseum) is an herb or shrub found in tropical and subtemperate regions of northwestern India. The plant grows, for example, in Jammu, Kashmir and Udhampur in India, where it usually blooms and bears fruit from April to June (see Chowdhary and Wadhwa, flora of himachal pradesh pp 181-1821; Chapman and Hail, dictionary of natural products, Vol.7 , 414, 1994. Vol.1, 457, 1994; Index Kewensis, Vol.1, 184.1895; Om, wild and cultivated plants of J & K andladakh, Page 23, 1997). [0003] A description of Argyrolobium roseum can be found in: FLORA OF WEST PAKISTAN, p. 385, vol. 100, by E. Nasirl; FLORA OF THEHIMALAYA, p. 94, Plate No. 323, d 460, Oleg Polunin , by Adam St...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/00A61KA61K36/48A61PA61P3/10
CPCY10S514/866A61K36/48A61P3/10
Inventor 奥姆·普拉卡什·古普塔扎比尔·艾哈迈德阿沙·巴加特库尔迪普·库马尔·古普塔苏克德夫·斯瓦米·汉达
Owner COUNCIL OF SCI & IND RES
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