Method for font printing-preparation of tissue slice, and fill-up of tissue chip

Abstract

A movable type printing type method for preparing and repariing the tissue chip includes sticking the chip substrate carrying organic adhesive onto the surface of wax block, slicing, sticking the sliced section onto glass plate, baking, cooling, and putting it in organic solvent to dissolve said adhesive and wax.

Description

1. Technical field [0001] The present invention relates to a histological slicing method, more particularly, relates to a slicing method utilizing carrier adhesion. 2. Background technology [0002] With the development of histology and pathology, people's understanding of the human body or other animals and plants has developed from the anatomical organ level to the tissue and cell level. However, the observation of tissues and cells needs to be carried out through microscope tools. However, since the complete organ is difficult to observe with a microscope, it is necessary to cut the organ into very thin sections for staining and observation. In order to solve this problem, a tissue section method has emerged. The principle of this method is to cut the organ into small pieces of tissue, fix it with formalin, use alcohol to remove the moisture in the tissue, then immerse it in xylene or benzene solution to remove the alcohol, and finally immerse the tissue piece in molten ...

AI Technical Summary

Problems solved by technology

The problem of "invalid tissue": Before the tissue chip is made, the tissue wax block must be diagnosed and marked. At this time, only the surface of the tissue is diagnosed and marked, and the structural changes in the deep tissue cannot be detected through the surface, such as a In the tumor tissue core, some are tumor tissue, some are tumor necrosis tissue called "ineffective tissue", fibers, blood vessel tissue, etc., which often cause some tissue points to be inconsistent with the original template design and original diagnosis. This phenomenon is almost It is unavoidable and can appear in all tumor tissues, and chips with too many "invalid tissues" can only be treated as waste chips

The problem of lack of displacement of sample tissue points: There are many procedures for making tissue microarrays, and the length of tissue cores is different, the tissue core and the acceptor wax block are not tightly combined, and the tissue core may shift or even fall off when slicing.

These two problems make the qualification rate of tissue chip products very low

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