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Immobilization glucose amylase products and method for immobilization of glucose amylase

A technology of glucoamylase and amylase enzymes, applied in biochemical equipment and methods, fixed on/in organic carriers, enzymes, etc., can solve the problems of poor carrier strength, easy rupture, enzyme molecule leakage, and glucoamylase immobilization It can achieve the effect of high apparent enzyme activity and good operability due to the lack of chemical methods

Inactive Publication Date: 2005-11-02
GANSU ACAD OF SCI INST OF BIOLOGY
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AI Technical Summary

Problems solved by technology

Among the above-mentioned carriers, after carrageenan, gelatin and sodium alginate embed glucoamylase, the strength of the carrier is poor and easy to break during use, resulting in the leakage of enzyme molecules
However, after polyacrylamide immobilized glucoamylase, although the apparent enzyme activity and the recovery rate of enzyme activity are high, the operability is poor, and the immobilization operation is complicated, and the cost of raw materials and preparation is also high.
[0005] At present, there are many reports on the immobilization of glucoamylase, but there are few immobilization methods of glucoamylase with polyvinyl alcohol as the carrier.

Method used

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  • Immobilization glucose amylase products and method for immobilization of glucose amylase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Take 50 mL of polyvinyl alcohol composite gel (containing dextran-40) with a concentration of 8%, and mix it with 5 mL of 10% glucoamylase (Wuxi Genenco Biological Engineering Co., Ltd., 50,000 units) enzyme solution ( Glucoamylase was dissolved with acetic acid-sodium acetate buffer solution of pH 4.6, filtered through multiple layers of gauze), mixed, stirred evenly, poured into a 20cm plate with a thickness of 3-4mm, and frozen at -24°C for 48 hours , take out after forming and thaw naturally, cut into small pieces of 3mm×3mm×3mm.

[0017] use Na 2 S 2 o 3 Standard solution titration (QB 1805.2-93 standard) was used to measure the activity of immobilized glucoamylase. At the same time, the starch hydrolysis experiment was carried out in batches, the DE value was determined, and the glucose content was determined by potassium ferricyanide titration. The results showed that the apparent enzyme activity of the immobilized glucoamylase was 1558u / g·dry·gel, the recove...

Embodiment 2

[0020] Repeat Example 1, with the following differences: use 50mL concentration of 10% polyvinyl alcohol composite gel, mix with 5mL concentration of 10% glucoamylase enzyme solution at room temperature, the apparent enzyme of the resulting immobilized glucoamylase The activity is 1480u / g·dry·gel.

[0021] Compared with Comparative Example A, due to the lower porosity and smaller pore size of the carrier, the continuous hydrolysis operation performance is slightly worse, and the production cost is also increased.

Embodiment 3

[0023] Repeat Example 1, with the following differences: the use of concentration is 10% polyvinyl alcohol composite gel 50mL, mixed with 10% glucoamylase enzyme solution at room temperature with 5mL concentration, stirred evenly, poured into a petri dish, thickness 1mm, Then freeze at -24°C for 48 hours, and cut into strips of 3mm×6mm after being shaped. The results showed that the apparent activity of the immobilized glucoamylase was 2573.6u / g·dry·gel, and the recovery rate of the enzyme activity was 51.2%.

[0024] Compared with "Example 1", its operability is worse than that of the granular immobilized enzyme, and it is easy to cause the enzyme molecule to fall off during operation.

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Abstract

The invention discloses an immobilization glucoamylase goods and a method of fixation of glucoamylase with polyvinyl alcohol composite gel. The immobilization glucoamylase goods is obtained from cryo-forming the mixture of polyvinyl alcohol composite gel and glucoamylase in low temperature. And the content of polyvinyl alcohol in polyvinyl alcohol composite gel is 8 to 15 percent(mass percent ), the concentration of glucoamylase is 8 to 15 percent(mass percent), Every 100ml composite gel can bear the weight of 0.5 to 1.5 gram of glucoamylase enzyme powder. The polyvinyl alcohol immobilizing glucoamylase goods has perfect mechanical capability, operating stabilization and has a good industrial application foreground. It can apply in alcohol industry, food industry, wine brewing, the finely processed of starch and starch stuff, analyzing and measuring, and other related ermentation industry.

Description

Technical field: [0001] The invention relates to an immobilized glucoamylase product and a method for immobilizing glucoamylase. Background technique: [0002] As an important biocatalyst, glucoamylase is widely used in starch syrup, glucose, brewing and fermentation industries. At present, free enzyme preparations are used in domestic industrial production, and there are difficulties in product separation, resulting in a huge waste of raw materials and increasing production costs. As a solid-phase biocatalyst, the immobilized glucoamylase product can not only separate the catalyst from the product (or substrate), but also realize continuous operation and automatic control, improving the quality and yield of the product. [0003] The immobilization methods of glucoamylase mainly include covalent bonding method, adsorption method, embedding method, co-immobilization and combination of several immobilization methods. Among them, the adsorption method is less used, mainly bec...

Claims

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Application Information

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IPC IPC(8): C12N11/04
Inventor 周剑平龚伟中魏甲乾王治业杨晖
Owner GANSU ACAD OF SCI INST OF BIOLOGY
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