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Trachyostracous mussel extract capable of increasing immunity function

A technology of thick-shelled mussels and extracts, which is applied in the directions of drug combination, medical raw materials from mollusks, medical raw materials from arthropods, etc.

Inactive Publication Date: 2005-10-19
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported that the enzymatic hydrolysis extracts of purple mussels and emerald mussels have anti-tumor, anti-oxidation, anti-coagulant, lowering blood pressure, lowering blood lipids, and improving immunity (Hong Pengzhi et al. Enzymatic hydrolysis of animal protein nutrition from emerald mussel meat Evaluation and Preliminary Exploration of its Physiological Activity Journal of Aquatic Sciences Vol.26, No.l, Feb., 2002; 5-4 Research on the Anti-tumor Effect of Shellfish Polysaccharides Hu Jianrao et al. CarcinogenesisTemtogenesis and Mutagenesis Vol.11, No.6 1999) but So far, there is no report about the alkali extract of mussel sclerosus improving immune function

Method used

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  • Trachyostracous mussel extract capable of increasing immunity function
  • Trachyostracous mussel extract capable of increasing immunity function
  • Trachyostracous mussel extract capable of increasing immunity function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1: Preparation of thick shell mussel extract

[0014] Wash thick-shelled mussels, remove their shells, mash their soft tissues with a mixer to homogenize; centrifuge to take 44.5 grams of precipitate, add 1 liter of 1% methanol to soak for 24 hours to remove fat, centrifuge to obtain the precipitate, add 1 liter of 1% NaOH to boil After 10 hours, the filtrate was filtered while hot to obtain 1240ml, and then 1 / 2 volume of n-butanol was added to extract three times. The extracted aqueous phase was concentrated to 600ml under reduced pressure with a rotary evaporator at 50°C, and 0.5% activated carbon was added for decolorization. Precipitate with 3 times the volume of ethanol overnight, discard the supernatant, and place the precipitate in an evaporating dish to dry naturally to obtain a block and powdery off-white solid 3.2g, add 250ml of double distilled water to dissolve in a boiling water bath, sevage method (chloroform and n-butanol The volume ratio is 4:1) Extr...

Embodiment 2

[0015] Example 2: Mouse Immunization Experiment

[0016] 1. Reagents: methanol, chloroform, n-butanol, glucose, phenol, acidified isopropanol, Indian ink, Na 2 CO 3 : Products of Huamei Reagent Company;

[0017] MTT, PHA, Wright's stain, NP 40 , Percoll separation solution: the product of Shenggong Biological Engineering Co., Ltd.;

[0018] Fresh sheep red blood cells (SRBC): Provided by the Department of Laboratory Medicine, Shanghai Changhai Hospital;

[0019] LDH kit: Nanjing Jiancheng Biological Reagent Co., Ltd. product;

[0020] Earles liquid: NaCl6.8g, KCl0.4g, NaH 2 PO 4 ·H 2 O0.14g, MgSO 4 ·7H 2 O0.2g, glucose 1g, 10% phenol red 1ml, add double distilled water to 100ml, then add 2% CaCl 2 10ml, add double distilled water to 1000ml, heat to dissolve, filter and separate, 8 lbs of autoclaving, store at 4℃;

[0021] Enterotoxic endotoxin: provided by the Central Laboratory of Shanghai Changhai Hospital;

[0022] Alsever solution: glucose 2.05g, NaCl0.42g, sodium citrate·H 2 ...

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Abstract

The present invention relates to immunological preparation, and a kind of trachyostracous mussel extract capable of raising immunity function. The trachyostracous mussel extract is the effective components extracted from the homogeneous slurry of soft trachyostracous mussel tissue. Animal test and cellular immunological experiment show that the trachyostracous mussel extract can increase the natural killer cell, macrophage and lymphocyte in immunity system, promote the formation of antibody and complement and strengthen the non-specific cell immunity function of body, so that it may be used in preparing immunity function raising preparation.

Description

Technical field [0001] The invention relates to the technical field of immune preparations, and is a thick-shell mussel extract capable of improving immune function. Background technique [0002] Thick-shelled mussel (Mytilus coruscus) is a kind of marine mollusk, rich in the seas around the Shengsi Islands in Zhejiang Province. It is rich in crude protein, fat, carbohydrates, in addition to calcium, phosphorus, iron, vitamins and other nutrients Substance, at the same time, mussels are a good medicine for kidney, with obvious functions of nourishing the kidney and strengthening yang, nourishing essence, nourishing blood, eliminating galls, and dispelling stagnation. It is known as "Yuan Dan" and was listed as a tribute to the court in the Ming and Qing Dynasties. "Gonggan" has now been living in harmony with the blue mussels that moved to the south, and can reproduce naturally in large numbers. The annual aquaculture output has reached nearly 20,000 tons...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/618A61P37/04
Inventor 冯伟华焦炳华张建鹏刘军华姚滢王俊魏江洲
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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