Polymorphic point of a1,2-fucose transferase gene mononucleotide for identifying resistances of hydrops and diarrhea of weaned piglets and use thereof
A fucose transferase, single nucleotide polymorphism technology, applied in the field of a1, can solve problems such as economic losses in the pig industry
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[0083] The self-constructed pig resource family with white Duroc and Chinese Erhualian pigs as ancestors and used for functional gene mapping was used as experimental animals. 479 boars of the second generation (F2 generation) of resource families were bred until they were slaughtered at 240 days old, and the small intestine and jejunum were taken to extract the brush border of small intestinal epithelial cells, and the brush border of small intestinal epithelial cells was extracted by using E. Microscopic examination of bacterial adhesion results. More than 20 brush borders were detected for each sample. If at least 2 bacteria adhered to more than 10% of the brush borders, the sample was judged as adherent type. If at least 2 bacteria adhered to the brush borders, less than 10 %, but there are more than 10% of brush borders with 1-2 bacterial adhesion, the sample is judged as weak adhesion type, otherwise it is judged as non-adhesion type. The adhesion effect picture is atta...
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