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Polymorphic point of a1,2-fucose transferase gene mononucleotide for identifying resistances of hydrops and diarrhea of weaned piglets and use thereof

A fucose transferase, single nucleotide polymorphism technology, applied in the field of a1, can solve problems such as economic losses in the pig industry

Inactive Publication Date: 2005-10-12
JIANGXI AGRICULTURAL UNIVERSITY
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  • Abstract
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Problems solved by technology

4-12 week old piglets have a morbidity rate of 30%-40% of pathogenic bacteria infection, and the mortality rate of sick piglets is 40%, sometimes even as high as 90%, which has caused great economic losses to the pig industry

Method used

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  • Polymorphic point of a1,2-fucose transferase gene mononucleotide for identifying resistances of hydrops and diarrhea of weaned piglets and use thereof
  • Polymorphic point of a1,2-fucose transferase gene mononucleotide for identifying resistances of hydrops and diarrhea of weaned piglets and use thereof
  • Polymorphic point of a1,2-fucose transferase gene mononucleotide for identifying resistances of hydrops and diarrhea of weaned piglets and use thereof

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Embodiment

[0083] The self-constructed pig resource family with white Duroc and Chinese Erhualian pigs as ancestors and used for functional gene mapping was used as experimental animals. 479 boars of the second generation (F2 generation) of resource families were bred until they were slaughtered at 240 days old, and the small intestine and jejunum were taken to extract the brush border of small intestinal epithelial cells, and the brush border of small intestinal epithelial cells was extracted by using E. Microscopic examination of bacterial adhesion results. More than 20 brush borders were detected for each sample. If at least 2 bacteria adhered to more than 10% of the brush borders, the sample was judged as adherent type. If at least 2 bacteria adhered to the brush borders, less than 10 %, but there are more than 10% of brush borders with 1-2 bacterial adhesion, the sample is judged as weak adhesion type, otherwise it is judged as non-adhesion type. The adhesion effect picture is atta...

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Abstract

A single nucleotide polymorphic locus of a1,2- fucose transfer enzyme gene affecting obviously resistant dropsy and diarrhea. It identifies single nucleotide polymorphic locus(SNP) c228T firstly and then judges type of SNPC 228T gene by way of PCR-SS CP,ARMS-PCR and so on.

Description

technical field [0001] The invention relates to a single nucleotide polymorphic site of a1,2-fucosyltransferase gene which can significantly affect the resistance of weaned piglets to edema and diarrhea and can be used for genetic improvement of breeding pigs. Background technique [0002] Edema and diarrhea in early weaned piglets is a major problem in current pig production. The prevention and treatment methods for this disease include vaccination, drugs, hygiene and isolation. However, these methods are not always effective, especially the use of antibiotics. Abuse causes animal products to carry a large amount of residual drugs and become drug food, which has adverse effects on human health. Therefore, it is urgent to find a safe, reliable and economically feasible prevention and control method. [0003] Imberchts et al. reported that enterotoxigenic Escherichia coli F18 (Enterotoxigenic escherichia coliF18, ECF18) is the main pathogen causing post-weaning edema and dia...

Claims

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Application Information

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IPC IPC(8): A01K67/00C12Q1/68
Inventor 黄路生晏学明舒青龙任军郭源梅麻骏武丁能水艾华水
Owner JIANGXI AGRICULTURAL UNIVERSITY
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