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Method for producing human lysozyme transgene cloning large-scale domestic animal as animal mammary gland bioreactor to recombine human lysozyme

A bioreactor, human lysozyme technology, which is applied in the fields of bioengineering and transgenic-cloning, can solve the problems such as no reports of human lysozyme transgenic cloning.

Inactive Publication Date: 2005-09-21
李宁
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0017] From the above-mentioned studies on human lysozyme transgene, it has not been reported that human lysozyme has been transgenicly cloned in large livestock

Method used

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  • Method for producing human lysozyme transgene cloning large-scale domestic animal as animal mammary gland bioreactor to recombine human lysozyme
  • Method for producing human lysozyme transgene cloning large-scale domestic animal as animal mammary gland bioreactor to recombine human lysozyme
  • Method for producing human lysozyme transgene cloning large-scale domestic animal as animal mammary gland bioreactor to recombine human lysozyme

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Experimental program
Comparison scheme
Effect test

Embodiment

[0088] 1 Experimental materials

[0089] 1.1 Plasmids and strains

[0090]Host bacteria Escherichia coli DH5α and DH10B, pBC1 milk expression vector kit and PCR-XL-TOPO vector were purchased from Invitrogen, pCMV-EGFP-IRES-NEO and pIRES-NEO were purchased from Clontech, pGEM-7zf was purchased from Promega, BAC RP11-1143G9 was purchased from American Gene Corporation

[0091] 1.2 Experimental animals

[0092] The 3-month-old fetuses of Holstein dairy cows were obtained from slaughterhouses, and the ovaries of yellow cattle were obtained from slaughterhouses in surrounding areas of Beijing.

[0093] 1.3 Main reagents

[0094] DMEM / F 12 Powder, DMEM powder medium, trypan blue (trypan blue), TCM199 powder, glutamine and G418 are products of Gibco Company; fetal bovine serum is product of Hyclone Company; dNTP and PCR primers were purchased from Shanghai Bioengineering Company; Taq enzyme was purchased from In the Agricultural Biotechnology Laboratory of China Agricultural Uni...

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Abstract

The invention relates to the animal galactophore biological reactor to reconstruct human antalzyme, a method to clone large livestock's by transudation the gene of the human antalzyme. It comprises the following steps:(1)construct an galactophore specifically expression carrier which includes human antalzyme genes; (2)construct double signs and single sign choice carrier, and get the gene cell through cell transudation by electronic; (3) Clone the body cell to get the transudation gene creature considering the cell as a nucleus. The cattle, created by transudation gene of human antalzyme which is an technique of clone with body cell, will produce a great deal of milk containing active human antalzyme. The human antalzyme produced by this invention, has the biological activity as the nature ones, and it could be developed into health milk with great appended value or purified the human antalzyme into medicine and health food.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to the field of transgene-cloning technology. Background technique [0002] The research on transgenic animals means that human beings change the genetic composition of animals purposefully, planned, based, and predictably according to their own wishes, and the purposes of changing their genetic composition are various. For example, geneticists hope to observe phenotypic changes by changing the genetic composition of animals, and physiologists hope to study the impact of a gene on the physiological state of the body through the expression of a specific gene. Transgenic research related to animal production hopes to endow animals with new phenotypic traits through transgenic technology. The research relies on molecular biology, animal embryo and gamete manipulation techniques in experimental techniques. [0003] At present, the production methods of transgenic animals mainly include m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/00
Inventor 汤波戴蕴平龚国春于政权张磊李宁
Owner 李宁
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