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Tagged primer chained to Pm21 gene of anti powdery mildew of wheat and application

A powdery mildew resistance gene and marker primer technology, which is applied in biochemical equipment and methods, microbial measurement/testing, fermentation, etc., can solve problems such as differences in product length, and achieve highly specialized effects

Inactive Publication Date: 2005-08-31
NANJING AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

Since such sequences have copies on homologous chromosomes and have high homology, the copies on different chromosomes will have common PCR primer binding sites; due to the long-term evolution of disease-resistant gene analogs Because of the change in different copy lengths, PCR products with the same primers will have different lengths

Method used

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  • Tagged primer chained to Pm21 gene of anti powdery mildew of wheat and application
  • Tagged primer chained to Pm21 gene of anti powdery mildew of wheat and application
  • Tagged primer chained to Pm21 gene of anti powdery mildew of wheat and application

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Embodiment Construction

[0038] 1. Screening of disease resistance gene analogues located on chromosome 6V

[0039] The seeds of T. villosa were sown in petri dishes to germinate, and then transplanted into pots after turning white. At the three-leaf stage, the mixed powdery mildew spores cultivated on the susceptible material Sumai No. 3 were shaken off on the seedlings. Materials induced by powdery mildew were sampled 24, 48, and 72 hours after inoculation, and RNA was extracted and mixed to form the experimental group; non-induced materials were sampled at the same time during these three periods, and RNA was extracted and mixed to form the control group. The experimental group and the control group were hybridized to the barley expression profile chip (purchased from Affymetrix Company) at the same time (the chip hybridization experiment was completed at the Shanghai National Biochip Center), and some probes that up-regulated expression were obtained, and one of the probes, Contig17515, was an anti...

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Abstract

A marker primer linked with the gene Pm21 resisting to powdery mildew of wheat features that the primers NAU / XiBao15F and NAU / XiBao 15R are designed according to the sequence of probe congtig 17515, and the experiments shown that it is linked with said gene Pm21. It can be used to culture the wheat resisting to powdery mildew and verify the purity of ordinary wheat variety.

Description

1. Technical field [0001] The invention discloses a marker primer linked with wheat powdery mildew resistance gene Pm21, which can be used for molecular marker-assisted selection in wheat powdery mildew resistance breeding; the marker can also be used to identify the purity of common wheat seeds, especially to identify the creation of cell engineering and chromosome engineering Chromosomal mutations involving common wheat homology group VI. 2. Technical Background [0002] Wheat (T. aestivum L), as an important food crop worldwide, plays a pivotal role in agricultural production, but its production is affected by many biotic and abiotic stresses. Wheat powdery mildew (Ergsiphe greminis f.sp.tritici), caused by the obligate parasitic fungus Erysiphegraminis DC, is one of the diseases that are increasingly harmful to wheat production in China and many countries in the world. improvement, and there has been an increasing trend in recent years. [0003] Common wheat (T. aestiv...

Claims

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Application Information

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IPC IPC(8): C12P19/34C12Q1/68
Inventor 曹爱忠陈佩度王秀娥张守忠王苏玲
Owner NANJING AGRICULTURAL UNIVERSITY
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