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Process for preparing glucose-reducing sand sagebrush polysaccharide and its use

The technology of Artemisia annua polysaccharide and Artemisia annua is applied in the directions of medical preparations containing active ingredients, pharmaceutical formulations, metabolic diseases, etc., and can solve problems such as preparation methods and applications of natural products that do not involve hypoglycemics.

Inactive Publication Date: 2005-08-17
NORTHWEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

None of the patents at home and abroad involve the preparation method and application of hypoglycemic natural products made from artemisia polysaccharides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Preparation method and application of hypoglycemic natural product from Artemisia annua seeds

[0016] Step 1: Artemisia annua seeds treated with CO 2 The part after supercritical extraction and degreasing was fully dissolved in water (the ratio of raw material to water was 1:30), ethanol (the ethanol content reached 50%) was added to the aqueous solution, left still, overnight, centrifuged, and the precipitate was collected and vacuum-dried to obtain substance A.

[0017] Step 2: Take a certain amount of material A and dissolve it in 80 times of water, extract it with a mixture of chloroform and n-butanol (1:2), add papain to the water solution for enzymolysis, and then use chloroform and n-butanol ( 1:2) mixture was extracted, and the collected aqueous solution was concentrated under reduced pressure and dried to obtain substance B. The ratio of A to the extract is 4:1, and the amount of papain is 24.00U / 2000ml aqueous solution.

[0018] Step 3: Pass substance B thr...

Embodiment 2

[0027] Preparation method and application of hypoglycemic natural product in Artemisia annua seeds

[0028] Step 1: Artemisia annua seeds treated with CO 2 The part after supercritical extraction and degreasing was fully dissolved in water (the ratio of raw material to water was 1:50), and ethanol was added to the aqueous solution (the ethanol content reached 80%) to stand still, overnight, and centrifuged, and the precipitate was collected and vacuum-dried to obtain substance A.

[0029]Step 2: Take a certain amount of substance A and dissolve it in 100 times of water, extract it with a mixture of chloroform and n-butanol (1:4), add papain to the water solution for enzymolysis, and then use chloroform and n-butanol ( 1:4) mixture was extracted, and the collected aqueous solution was concentrated under reduced pressure and dried to obtain substance B. The ratio of A to the extract is 4:1, and the amount of papain is 24.00U / 2000ml aqueous solution.

[0030] Step 3: Pass subst...

Embodiment 3

[0033] Preparation method and application of hypoglycemic natural product from Artemisia annua seeds

[0034] Step 1: Artemisia annua seeds treated with CO 2 The part after supercritical extraction and degreasing was fully dissolved in water (the ratio of raw material to water was 1:40), and ethanol (the ethanol content reached 70%) was added to the aqueous solution to stand still, overnight, centrifuged, and the precipitate was collected and vacuum-dried to obtain substance A.

[0035] Step 2: Take a certain amount of material A and dissolve it in 90 times of water, extract it with a mixture of chloroform and n-butanol (1:3), add papain to the water solution for enzymolysis, and then use chloroform and n-butanol ( 1:3) mixture was extracted, and the collected aqueous solution was concentrated under reduced pressure and dried to obtain substance B. The ratio of A to the extract is 4:1, and the amount of papain is 24.00U / 2000ml aqueous solution.

[0036] Step 3: Pass substanc...

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Abstract

The present invention is sand sage polysaccharide, prepared with sand sage seed deoiling dreg as material and through extraction, separation, enzymolysis and chromatographic separation, and its preparation process and application. The preparation process features 50-80 concentration alcohol solution extraction, extraction with mixed chloroform and normal butyl alcohol liquid, enzymolysis with papain, and chromatographic separation in sephadex G-20 column. Sand sage polysaccharide is used in treating alloxan diabetes, reducing the damage of liver and kidney caused by diabetes, lowering the content of glutamic-oxaloacetic transminase, glutamic-pyruvic transminase and urea nitrogen in blood, and inhibiting diabetes caused weight loss.

Description

technical field [0001] The invention relates to a method for preparing Artemisia annua polysaccharide with hypoglycemic effect, which is prepared by extracting, separating, enzymatically hydrolyzing and column chromatography using the waste residue after removing oil from natural Artemisia annua seeds and its application. Background technique [0002] Diabetes mellitus is a metabolic disorder syndrome characterized by chronic hyperglycemia associated with genetic factors and various environmental factors. It is a common disease that seriously endangers human health worldwide. In recent years, the prevalence and mortality of diabetes in the world have shown a gradual upward trend, and it has become one of the key issues to be solved and overcome in the world's medical field today. Commonly used hypoglycemic drugs are mainly synthetic drugs, which have certain damage to the liver and kidney and are not easily accepted by patients. [0003] Patent JP95 / 00560 applied for a new ...

Claims

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Application Information

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IPC IPC(8): A61K31/715A61P3/10C08B37/00
Inventor 张继侯天德王云普姚健程昉曾家豫吴建黄玉龙马君义王振恒李芳成
Owner NORTHWEST NORMAL UNIVERSITY
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