Process for preparing glucose-reducing sand sagebrush polysaccharide and its use
The technology of Artemisia annua polysaccharide and Artemisia annua is applied in the directions of medical preparations containing active ingredients, pharmaceutical formulations, metabolic diseases, etc., and can solve problems such as preparation methods and applications of natural products that do not involve hypoglycemics.
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Embodiment 1
[0015] Preparation method and application of hypoglycemic natural product from Artemisia annua seeds
[0016] Step 1: Artemisia annua seeds treated with CO 2 The part after supercritical extraction and degreasing was fully dissolved in water (the ratio of raw material to water was 1:30), ethanol (the ethanol content reached 50%) was added to the aqueous solution, left still, overnight, centrifuged, and the precipitate was collected and vacuum-dried to obtain substance A.
[0017] Step 2: Take a certain amount of material A and dissolve it in 80 times of water, extract it with a mixture of chloroform and n-butanol (1:2), add papain to the water solution for enzymolysis, and then use chloroform and n-butanol ( 1:2) mixture was extracted, and the collected aqueous solution was concentrated under reduced pressure and dried to obtain substance B. The ratio of A to the extract is 4:1, and the amount of papain is 24.00U / 2000ml aqueous solution.
[0018] Step 3: Pass substance B thr...
Embodiment 2
[0027] Preparation method and application of hypoglycemic natural product in Artemisia annua seeds
[0028] Step 1: Artemisia annua seeds treated with CO 2 The part after supercritical extraction and degreasing was fully dissolved in water (the ratio of raw material to water was 1:50), and ethanol was added to the aqueous solution (the ethanol content reached 80%) to stand still, overnight, and centrifuged, and the precipitate was collected and vacuum-dried to obtain substance A.
[0029]Step 2: Take a certain amount of substance A and dissolve it in 100 times of water, extract it with a mixture of chloroform and n-butanol (1:4), add papain to the water solution for enzymolysis, and then use chloroform and n-butanol ( 1:4) mixture was extracted, and the collected aqueous solution was concentrated under reduced pressure and dried to obtain substance B. The ratio of A to the extract is 4:1, and the amount of papain is 24.00U / 2000ml aqueous solution.
[0030] Step 3: Pass subst...
Embodiment 3
[0033] Preparation method and application of hypoglycemic natural product from Artemisia annua seeds
[0034] Step 1: Artemisia annua seeds treated with CO 2 The part after supercritical extraction and degreasing was fully dissolved in water (the ratio of raw material to water was 1:40), and ethanol (the ethanol content reached 70%) was added to the aqueous solution to stand still, overnight, centrifuged, and the precipitate was collected and vacuum-dried to obtain substance A.
[0035] Step 2: Take a certain amount of material A and dissolve it in 90 times of water, extract it with a mixture of chloroform and n-butanol (1:3), add papain to the water solution for enzymolysis, and then use chloroform and n-butanol ( 1:3) mixture was extracted, and the collected aqueous solution was concentrated under reduced pressure and dried to obtain substance B. The ratio of A to the extract is 4:1, and the amount of papain is 24.00U / 2000ml aqueous solution.
[0036] Step 3: Pass substanc...
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