Compound extracted from eucalyptus plants and use thereof

A technology of compounds and uses, applied in the field of application in the treatment of tumors, can solve problems such as insufficient research

Inactive Publication Date: 2005-07-06
中国人民解放军第二军医大学药学院 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the useful compounds in Eucalyptus are not well studied so far

Method used

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  • Compound extracted from eucalyptus plants and use thereof
  • Compound extracted from eucalyptus plants and use thereof
  • Compound extracted from eucalyptus plants and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] New compound extracted from eucalyptus

[0068] A. Extracted from blue eucalyptus fruit

[0069] (A1) Extraction process

[0070] Extract as follows:

[0071] 1. Take the pulverized medicinal material (the fruit of Eucalyptus blueus, commonly known as a bell), extract it with 95% ethanol at room temperature for two weeks, and reclaim the ethanol solution to obtain the ethanol extract.

[0072] 2. Take the ethanol extract, extract it with petroleum ether first, then extract it with ethyl acetate, and recover the ethyl acetate solvent to obtain the ethyl acetate part.

[0073] 3. Take an appropriate amount of ethyl acetate and separate it on a silica gel column. The elution gradient is petroleum ether: ethyl acetate = 10:1, petroleum ether: ethyl acetate = 5:1, petroleum ether: ethyl acetate = 3:1, petroleum ether: ethyl acetate = 2:1

[0074] 4. Collect the fraction eluted with petroleum ether: ethyl acetate = 2:1, and concentrate to obtain a brown-red viscous substa...

Embodiment 2

[0098] Study on anti-tumor effect in vitro

[0099] Huh-7 human liver cancer cell line, Du-145 human prostate cancer cell line, K562 human leukemia cell line, AGS human gastric cancer cell line, Eca-109 human esophageal cancer cell line, 786-0 human kidney cancer cell line, A549 human Lung cancer cell lines and CS-174-T human colon cancer cell lines were used for drug anti-tumor experiments in vitro. Cells were cultured in culture flasks until the number of cells reached 1×10 7 indivual. After the cells were digested, the cells were diluted to 3×10 with DMEM medium (GIBCO Company) 4 / ml. Take a 96-well plate, add 200ul diluted cell solution to each well, and store at 37°C in 5% CO 2 Incubation in the incubator. After 24 hours, discard the original cell culture medium, add 200ul of solutions containing different concentrations of various test drugs prepared with DMEM culture medium to the corresponding wells, and then continue to place the cell culture plate at 37°C in 5% ...

Embodiment 3

[0104] Study on anti-tumor effect in vivo

[0105] Huh-7 human liver cancer cells were routinely cultured. Take cells with good activity in the proliferative phase, with a volume of 0.25ml and a concentration of 1×10 7 The dose of cells / mouse was inoculated subcutaneously on the outer right thigh of nude mice (purchased from Shanghai Experimental Animal Center, Chinese Academy of Sciences). The next day after inoculation, the mice were randomly divided into 2 groups, 10 in each group. After raising for 30 days, give each mouse of the test drug group intraperitoneal injection of medicine (100mg / kg) solution 0.5ml every day; each mouse of the negative control group is given equal volume of normal saline every day; each group of mice is given 1 dose every day times, continuous administration for 10 days. After 24 hours of drug withdrawal, the mice in each group were sacrificed by bleeding, autopsy was performed, the tumor mass was taken out and weighed, and the tumor inhibitio...

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PUM

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Abstract

The invention discloses a tricyclic sesquiterpene compound extracted from eucalyptus, a pharmaceutical composition containing the compound and its application in treating tumors. In vitro and animal experiments have proved that this tricyclic sesquiterpene compound extracted from Eucalyptus can inhibit the growth of liver cancer, gastric cancer, esophageal cancer, prostate cancer, kidney cancer, lung cancer and colon cancer cell lines. Mice inoculated with tumors also had an inhibitory effect.

Description

technical field [0001] The invention relates to a tricyclic sesquiterpene compound extracted from eucalyptus, a pharmaceutical composition containing the compound and its application in treating tumors. Background technique [0002] Eucalyptus is a main genus and species of Myrtaceae. It is native to Australia and has been introduced into China since 1890. At present, it has been planted on a large scale in my country and has become one of the three major afforestation tree species in my country. [0003] Blue eucalyptus is a major member of the genus Eucalyptus, also known as gray willow, eucalyptus, small ball eucalyptus, blue oil wood, poplar fruit tree. [0004] The original main uses of Eucalyptus plants are as follows: their stems and branches can be used to make wood, sleepers, packaging materials and papermaking; the eucalyptus oil extracted from its leaves can be used in spice production and medicinal purposes; Eucalyptus oil is mostly blue eucalyptus oil. [0005...

Claims

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Application Information

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IPC IPC(8): A61K31/11A61P35/00C07C45/78C07C47/565
Inventor 娄子洋刘玉明吴玉田朱洪莉戈萌谢天培
Owner 中国人民解放军第二军医大学药学院
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