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Ultrasound contrast medium composition with phospholipid as membrane material and its preparation method

A technology of ultrasonic contrast agent and film-forming material, which is applied in the direction of echo/ultrasonic imaging agent, etc., to achieve the effect of good microbubble uniformity, low product cost and high output rate

Inactive Publication Date: 2005-06-29
INST OF PHARMACOLOGY & TOXICOLOGY ACAD OF MILITARY MEDICAL SCI P L A +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The phospholipid film that constitutes the phospholipid contrast agent is biodegradable and harmless to the human body; however, the albumin contrast agent still has the risk of spreading blood diseases because it uses human albumin as the carrier

Method used

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  • Ultrasound contrast medium composition with phospholipid as membrane material and its preparation method
  • Ultrasound contrast medium composition with phospholipid as membrane material and its preparation method
  • Ultrasound contrast medium composition with phospholipid as membrane material and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Preparation of a contrast agent using phospholipids as a film-forming material

[0028] Weigh 1.5 mg palmitic acid, 2 mg glyceryl monostearate, 120 mg polyethylene glycol 1500 (PEG1500) and 120 mg Poloxamer 188 (Poloxamer 188) and disperse them in 3 ml of tert-butanol, dissolve in a water bath at 60°C. Separately weigh 20 mg of egg yolk lecithin (PC) and disperse it in 1 ml of tert-butanol, ultrasonically dissolve it in a water bath at 60 °C, mix it with the upper liquid, and quickly cool it at 0-4 °C for more than 30 minutes. Hour. Weigh 200 mg of the freeze-dried sample and put it in a 5 ml vial, fill it with perfluoropropane gas, and seal it tightly to prepare a solid ultrasound contrast agent.

[0029] Inject 2ml of normal saline into the stopper when using it, shake gently to form a microbubble suspension, and take a sample to observe that the microbubble concentration is 2×10 7 / ml, the particle size distribution is 2-10um, and the cell wall thickness...

Embodiment 2

[0031] Example 2: Effect of Tween

[0032] Weigh 1.5 mg palmitic acid, 2 mg glyceryl monostearate, 120 mg polyethylene glycol 1500 (PEG1500) and 120 mg Poloxamer 188 (Poloxamer 188) and disperse them in 3 ml of tert-butanol, dissolve in a water bath at 60°C. Separately weigh 20 mg of egg yolk lecithin (PC) and different amounts of Tween 80 (Tween 80) and disperse them in 1 ml of tert-butanol, ultrasonically dissolve them in a water bath at 60°C, mix them with the supernatant, and quickly place them in a cold place at 0-4°C for 30 More than 10 minutes, the liquid is solidified and freeze-dried for 20 hours. Weigh 200 mg of the freeze-dried sample and put it in a 5 ml vial, fill it with perfluoropropane gas, and seal it tightly to prepare a solid ultrasound contrast agent.

[0033] Inject 2ml of normal saline into the stopper when using it, shake gently to form a microbubble suspension, take samples to observe the concentration, average particle size and percentage of microbubb...

Embodiment 3

[0046] Embodiment 3: the impact after hydrogenation of lecithin

[0047] Studies have shown that long-circulating liposomes prepared with hydrogenated egg phosphatidylcholine (HEPC) are cleared slower than long-circulating liposomes prepared with non-hydrogenated egg phosphatidylcholine (EPC). We hydrogenate egg yolk lecithin: powdered egg yolk lecithin (PC) is dissolved in ethanol, added with 5% palladium carbon, and hydrogenated on a hydrogenator. After the hydrogenation treatment, filter, spin the filtrate under reduced pressure to remove ethanol, and freeze-dry to obtain white hydrogenated egg phosphatidylcholine (HEPC).

[0048] Substitute 20 mg of egg yolk lecithin (PC) in Example 1 with 5 mg of hydrogenated lecithin, and 25 mg of Tween 80. The rest of the steps were the same as in Example 1 to prepare an ultrasound contrast agent. Sampling and observing the microbubble concentration was 1×10 8 / ml, the particle size distribution is 2-8um, and the cell wall thickness i...

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Abstract

The invention relates to an ultrasound contrast medium composition with phospholipid as membrane material and its preparation method, wherein the contrast medium comprises film forming material and fluorine-carbon type inert gases, the film forming material comprises phosphatide constituent 1-10%, foaming agent 5-15%, stabilizing agent 0.5-10%, and polymeric compound 70-90%.

Description

Technical field: [0001] The invention relates to an ultrasonic contrast agent composition, in particular to an ultrasonic contrast agent composition which uses phospholipid components as a film-forming material and wraps fluorocarbon inert gas and a preparation method thereof. technical background: [0002] The new ultrasound contrast agent combined with the new ultrasound technology can effectively enhance the two-dimensional ultrasound images and blood flow Doppler signals of the myocardium, liver, kidney, brain and other solid organs, reflecting the different blood patterns of normal tissues and diseased tissues (tumor, ischemic myocardium). Flow perfusion significantly improves the sensitivity and specificity of ultrasound diagnosis. In addition, ultrasound contrast agents carrying genes and drugs also have broad application prospects in treatment. The ideal new ultrasound contrast agent has the following characteristics: high scattering, low diffusibility, low solubili...

Claims

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Application Information

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IPC IPC(8): A61K49/22
Inventor 梅兴国李思成赵应征唐杰张彦栾新慧
Owner INST OF PHARMACOLOGY & TOXICOLOGY ACAD OF MILITARY MEDICAL SCI P L A
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