Method for preparing low molecular weight seaweed polysaccharide sulfate

Abstract

The present invention relates to the preparation process of low molecular weight seaweed polysaccharide sulfate. The preparation process includes adding ascorbic acid and hydrogen peroxide into the solution of natural seaweed polysaccharide sulfate as material, with the added amount being 0.1-50 mmol / L and 0.1-100 mmol / L and the ratio being 1 to 0.1-10, constant degradation under controlled temperature for 0.5-3 hr, dialysis or ultrafiltering, decompression concentration and freeze drying to obtain low molecular weight seaweed polysaccharide sulfate product of molecular weight 4-100 KDa. The present invention has the advantages of fast degradation speed, low reagent consumption, simple process, concentrated molecular weight distribution and other advantages. The low molecular weight seaweed polysaccharide sulfate product has controllable molecular weight, greatly raised dissolubility and bioactivity.

Description

technical field [0001] The invention relates to the research and development of marine medicines, in particular to a preparation method of low-molecular-weight seaweed sulfated polysaccharides, which belongs to the technical field of marine biology. Background technique [0002] Sulfated polysaccharides are polysaccharides in which one or more hydroxyl positions in the polysaccharide constituent units are sulfated. In the plant kingdom, only algae have natural sulfated polysaccharides. Seaweed sulfated polysaccharide is a kind of polysaccharide unique to seaweed. Seaweed sulfated polysaccharides have a wide range of biological activities, including antiviral, antitumor, growth factor effects, immune system effects, and anticoagulant activity. It has been reported that fucoidan sulfate and carrageenan have anti-HIV-1 cytotoxic activity (CD4 + T lymphocytes), can inhibit HIV-1 on CD4 + Infection of the lymphoid cell line MT-4. In addition, seaweed sulfated polysaccharides...

AI Technical Summary

Problems solved by technology

[0003] The current problem is that most of the natural seaweed sulfated polysaccharides are polysaccharides with large molecular weight

[0006] 2. Alkaline hydrolysis, under alkaline conditions, often causes the modification of acidic polysaccharides and the shedding of sulfate groups, which affects the activity of the product, so it is not suitable for seaweed sulfated polysaccharides

These two methods are currently unable to be applied to industrial production due to high energy consumption, high requirements for equipment conditions, and small sample processing volume.

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