Gel column mounting method

Abstract

The present invention provides a gel column mounting method. Gel slurry is first heated and boiled, and then transferred with improved slender transfer pipet into rotating gel pipe along the inner wall of gel pipe under liquid surface, so as to prepare gel column. By means of boiling, deairing and transferring gel slurry under liquid surface, the present invention can defoam, swell and sterilize. The gel slurry has flat surface during being transferred into the gel pipe. The method is simple, saving in time and convenient, and has no need of special apparatus and column mounting success rate up to 100 %.

Description

technical field [0001] The invention relates to a chromatographic separation device, in particular to a new method for packing a gel column. Background technique [0002] Gel chromatography is a fast and simple separation and grading method for water-soluble substances. It is widely used in biochemistry, molecular biology and medicine and other related fields. An indispensable technical means for macromolecular substances. The traditional column packing method is divided into mechanical column packing method and manual column packing method. The mechanical column packing method uses a peristaltic pump to slowly add the prepared gel slurry into the column. Generally, the quality is good, but it is limited by equipment conditions. Especially in the laboratory, it cannot be widely used; the manual column packing method is simple and does not require special tools, but the traditional manual column packing method has two defects. The air dissolved inside the gel particles and ...

AI Technical Summary

Problems solved by technology

The traditional column packing method is divided into mechanical column packing method and manual column packing method. The mechanical column packing method uses a peristaltic pump to slowly add the prepared gel slurry into the column. Generally, the quality is good, but it is limited by equipment conditions. Especially in the laboratory, it cannot be widely used; the manual column packing method is simple and does not require special tools, but the traditional manual column packing method has two defects. The air dissolved inside the gel particles and in the gel slurry is completely removed. In addition, the gas is easily trapped during the transfer of the gel slurry to the chromatography column, so that air bubbles are easy to form in the gel column; the second is that the addition of the gel slurry Discontinuous, or when the gel slurry is added to the wall, the larger particles will settle on the side of the chromatography column with a higher flow rate, resulting in an uneven gel bed, which will cause cracks in the gel column bed and affect the chromatography. Therefore, rich column packing experience is required, which is difficult for beginners to master, and often cannot be successfully packed at one time.

In addition, due to the characteristics of the gel particles themselves, after the gel column is used for a period of time, the gel particles will be gradually compacted, and eventually cannot be used any longer. The column needs to be repacked frequently, and the work intensity is very high.