Peptide feed additive for increasing quality of fowls egg, its preparation method and use
A technology of feed additives and peptides, applied in the field of peptide feed additives and its preparation, can solve the problems of changing the quality of poultry eggs, achieve the effects of improving utilization rate, increasing egg production rate, and promoting digestion
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Embodiment 1
[0064] The Bacillus subtilis was inserted into the sterilized liquid culture medium and cultured at 25°C for 48 hours to obtain a seed liquid.
[0065] The liquid culture medium is a mixture prepared in the following proportions: peptone 1.3g, glucose 3g, molasses 3g, potato extract 50ml, soluble starch 10g, sucrose 10g, beef extract 2g, yeast extract powder 3.5g, soybean meal 1.5 g, growth factor 1ml, water 1000ml; the growth factor contains MgSO 4 .7H 2 O 20.00mg / ml, CaCl 2 .2H 2 O2.20mg / ml, ZnSO 4 .7H 2 O 0.81mg / ml, FeSO 4 .7H 2 O 0.44mg / ml, MnSO 4 .H 2 O 0.13mg / ml, CuSO 4 .5H 2 O 0.02mg / ml mixture.
[0066] The fermentation substrate-soy protein isolate powder is crushed to 40 meshes and put into the fermenter, water and carbon source-corn flour and molasses (weight ratio 1:1) are added in sequence, and the weight of the added water is compared with the fermentation substrate The weight ratio of the protein contained is 100:3, the weight ratio of the carbon content of the adde...
Embodiment 2
[0088] The Aspergillus niger was inserted into the sterilized liquid medium and cultivated at 35°C for 24 hours to obtain the seed liquid.
[0089] The liquid medium is a mixture prepared in the following proportions: peptone 2.5g, glucose 5g, molasses 5g, potato extract 100ml, soluble starch 20g, sucrose 30g, beef extract 3g, yeast extract powder 5g, soybean meal 2g, Growth factor 2ml, water 1000ml; the growth factor contains MgSO 4 .7H 2 O 30.00mg / ml, CaCl 2 .2H 2 O3.50mg / ml, ZnSO 4 .7H 2 O 1.10mg / ml, FeSO 4 .7H 2 O 0.60mg / ml, MnSO 4 .H 2 O 0.20mg / ml, CuSO 4 .5H 2 O 0.04mg / ml mixture.
[0090] The fermented substrate-bean cake is crushed to 120 meshes and put into the fermenter, water and carbon source-corn molasses and white sugar (weight ratio = 3: 2) are added in sequence, and the weight of the added water is determined by the weight of the fermented substrate. The weight ratio of protein content is 100:20, the weight ratio of the carbon content of the added carbon source to ...
Embodiment 3
[0092] The Saccharomonas viridans was added to the sterilized liquid medium and cultured at 27° C. for 36 hours to obtain the seed liquid.
[0093] The liquid culture medium is a mixed solution prepared in the following proportions: 1.8g peptone, 4g glucose, 4g molasses, 70ml potato extract, 15g soluble starch, 20g sucrose, 2.5g beef extract, 4g yeast extract, 1.8g soybean meal g, 1.5ml growth factor, 1000ml water; the growth factor contains MgSO 4 .7H 2 O 25.00mg / ml, CaCl 2 .2H 2 O 3.00mg / ml, ZnSO 4 .7H 2 O 1.00mg / ml, FeSO 4 .7H 2 O 0.50mg / ml, MnSO 4 .H 2 O0.17mg / ml, CuSO 4 .5H 2 O 0.03mg / ml mixed solution.
[0094] The fermentation substrate-soybean meal is crushed to 80 mesh and put into the fermentor, and water and carbon source-starch are added in sequence. The weight ratio of the added water to the protein contained in the fermentation substrate is 100:10. The weight ratio of the carbon content of the carbon source to the nitrogen content of the fermentation substrate is 1:1...
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