White flower beet the 9th chromosome BAC chip and its preparation method

Abstract

A white bloom beet chromosome 9 BAC chip and its preparing method are provided. BAC clone on the BAC chip is obtained by screening the BAC library using white bloom beet chromosome, and the chip is prepared by combining the BAC clone with the carrier. The preparing method comprises: primary treatment for BAC, BAC treatment before spotting, and preparation of the BAC chip. M14 and M14 chromosome addition line differential expression gene screening, genome differential analysis, gene mapping and number of replications analysis can be done rapidly and efficiently by using the chip prepared by the method.

Description

Technical field: [0001] The present invention relates to the field of biotechnology, and in particular to a BAC chip of chromosome 9 of beet beet and a preparation method thereof. Background technique: [0002] Plant apomixis (Apomixis) refers to a special asexual reproduction method that reproduces by seeds without fusion of sperm and eggs. Since there is no diploid offspring produced by the fusion of sperm and egg, their genotype is exactly the same as that of the mother, so that the offspring inherit the inherent genetic characteristics of the parent and form a genetically stable clone that can be reproduced by seeds [2]. Plant apomixis because of its special asexual reproduction through seeds, it can fix favorable genotypes, change breeding procedures and seed production, which has great economic significance. If it can be applied to important crops, it will be a successor to the sixties of the twentieth century. After the "Green Revolution" in the 1980s, another agricultural...

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Problems solved by technology

On the other hand, the density limit of the membrane chip requires the same probe to hybridize dozens of membranes, which leads to incomparability of signal values ​​between different membranes.