Method for lanthanon promoting saffron cell growth and increasing saffron essence content

A rare earth element and crocin technology, applied in the directions of botanical equipment and methods, plant cells, horticultural methods, etc., can solve problems such as shortage of crocin resources, and achieve the effects of increasing crocin content, not easy to brown, and promoting growth

Inactive Publication Date: 2005-01-26
INST OF PROCESS ENG CHINESE ACAD OF SCI
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Problems solved by technology

This method provides the basis for the large-scale production of crocin by using the plant hormones of rare eart...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Wash the earth on the surface of the saffron corm with tap water, remove the skin film, if there are scabs, remove the scabs with a scalpel, then rinse 2 times with distilled water, dry them through filter paper, soak for 30 seconds with 70wt% alcohol, use 2wt% active Disinfect with sodium hypochlorite solution of chlorine for 15 minutes, then wash 5 times with sterile water, finally blot the moisture on the bulb surface with sterile filter paper, cut the washed bulb into small pieces of about 1 square centimeter with a scalpel, and inoculate them in On MS plant medium containing 0.25mg / g 2,4-D, and 2.0mg / g 6-BA plant hormones, placed in an artificial climate box at 21±0.3°C or in a cultivation room at 27±1°C for 14-20 days , the bulb callus of saffron can be obtained.

[0022] The saffron bulb callus that forms will be inoculated on the MS plant cell culture medium that contains 0.05mM La, and add 2.0mg / g 2,4-D (being 2,4-dichlorophenoxyacetic acid) and 0.5mg / g 6-BA (...

Embodiment 2

[0026] The callus that the saffron corm that obtains in embodiment 1 forms is inoculated to containing 0.005mM La, 0.5mg / gNAA (being naphthalene acetic acid), and on the MS medium of 0.5mg / g 6-BA, cultivates 25 at 30 ℃. days, the biomass of the obtained callus increased by 25-36% compared with the control group without adding rare earth elements.

[0027] The callus after 25 days of culture was transferred to the B5 medium containing 0.02mM Ce, 0.5mg / g 2,4-D and 2mg / g 6-BA, and cultured at 15°C for 25 days, the content ratio of crocin was The control group increased by 40-50%.

Embodiment 3

[0029] The method for inducing saffron to form callus by the method of embodiment 1.

[0030] The callus that the flower of saffron is formed is inoculated to containing 0.015mM mixed rare earth element (its composition and mol ratio are La 2 o 3 : CeO 2 :Pr 6 o 11 :Sm 2 o 3 =255:175:3:10), 0.5mg / gNAA, and 0.5mg / g6-BA on MS medium cultured at 18°C ​​for 25 days, the biomass of the callus obtained was higher than that of the control group without rare earth elements Increased by 30-36%.

[0031] The callus after 25 days of culture was transferred to the B5 medium containing 0.03mM Ce, 2mg / g NAA and 0.5mg / g 6-BA, and cultured at 18°C ​​for 25 days, the content of crocin increased by 60% compared with the control group. ~80%.

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Abstract

The invention relates to a method for promoting saffron growth and increasing crocin content by the application of rare earth element. It inoculates the saffron callus to the vegetable cell medium containing rare earth elements, cytomin and archusia, cultures for 15 to 30 days under 15DEG C-30DEG C, then again inoculates the callus to the vegetable cell medium containing rare earth elements, cytomin and archusia for culturing 15 to 30 days under 15DEG C-30DEG C. It can promote the saffron cell growth and obtain more saffron cell biomass in the same culturing time compared with the prior part. The crocin content is markedly increased and the crocin obtained does not easy to turn brown.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for promoting the growth of saffron cells and increasing the content of saffron by using rare earth elements. technical background [0002] Saffron is a perennial herb of the Iridaceae (Iridaceae) genus Saffron, also known as saffron and saffron. It is native to Spain, Greece, southern European countries, Iran and other places. It was introduced into my country's Tibet via India, and is currently cultivated in my country's Zhejiang, Jiangsu, Shandong, Beijing and other places (Chengdu College of Traditional Chinese Medicine, Chinese Materia Medica Appraisal, 1977, 271-273 ; Jiangsu College of Traditional Chinese Medicine, Dictionary of Chinese Medicine (Volume 2) 1977: 2671). On the one hand, saffron (Saffron, Crocussativus L.) has high medicinal value in traditional and modern medicine. Studies have shown that saffron can be used for sedation, expectoration, sti...

Claims

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Application Information

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IPC IPC(8): A01G7/00A01H4/00C12N5/04
Inventor 王玉春陈书安王晓东赵兵袁晓凡
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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