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High subharmonic soft x-ray lining microscope

A phase-contrast microscope and high-order harmonic technology, applied in the field of microscopy, can solve problems such as ultra-fine structures that limit dynamic processes, and achieve the effect of high spatial coherence

Inactive Publication Date: 2005-01-12
SHANGHAI INST OF OPTICS & FINE MECHANICS CHINESE ACAD OF SCI
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Problems solved by technology

Unfortunately, the X-ray sources used in X-ray phase-contrast imaging are all X-ray tubes of synchrotron radiation sources, and the images are enlarged after recording, which limits its understanding of dynamic processes and ultrafine Structural research with a resolution of only about 10 μm

Method used

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  • High subharmonic soft x-ray lining microscope
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  • High subharmonic soft x-ray lining microscope

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Embodiment Construction

[0032] see first figure 1 , figure 1 It is a schematic diagram of an embodiment of a high-order harmonic soft X-ray phase contrast microscope of the present invention, which is composed of eight parts: a femtosecond laser 1, a converging lens 2, a hollow capillary waveguide sample chamber 4, a zone plate 5, and a detector 6 , computer 7 and vacuum chamber 8. In this example:

[0033] Said femtosecond laser 1 is a femtosecond Ti:Sapphire laser system with a pulse width of 20 fs, an output energy of 100 μJ, and a radiation wavelength of 800 nm.

[0034] Said converging lens 2 is an achromatic glass lens with a focal length of 100 mm and a diameter of 80 mm.

[0035] Said hollow capillary waveguide 3 has an inner diameter of 150 μm, a length of 64 mm, and is filled with helium.

[0036] Said sample chamber 4 is a working chamber for placing biological samples.

[0037] Said zone plate 5 is a Fresnel zone plate with a diameter of 3 mm, a focal length of 4 mm, and a number of ...

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Abstract

The phase contrast microscope includes femtosecond laser, and convergent lens, hollow capillary wave-guide, sample cell, zone plate and detector arranged in sequence on optical path. Capillary wave-guide, sample cell, zone plate and detector are setup inside vacuum chamber. The detector though wire is connected to computer outside the chamber. Laser through convergent lens is focused on capillary wave-guide, causing ionization of gas in capillary wave-guide to generate high order harmonic soft X-ray. The said soft X ray irradiates sample to be tested inside sample room. Then zone plate between sample room and detector forms a magnified image on the detector, which sends phase contrast image to computer for processing. The device possesses higher resolution and real time processing capability, being applicable to study ultra quick physical process of superfine structure.

Description

Technical field: [0001] The invention relates to a microscope, in particular to a high-order harmonic soft X-ray phase contrast microscope, which has extremely wide applications in biomedicine. Background technique: [0002] In recent years, due to the development of ultra-intense and ultra-short lasers, high-order harmonic radiation has entered the water window band, and this huge progress has attracted widespread attention. Ultrashort refers to the extreme time scale, the laser pulse width is only a few femtoseconds, and the distance that light travels within one femtosecond is 0.3μm, and superpower refers to extremely high power and power density. Currently, a laser system can produce a peak power of 1015W, and the focused light intensity can reach 10 21 ~10 22 W / cm 2 , the electric field generated by it is much larger than the Coulomb field inside the atom, and it can also generate 10 4 T's super strong magnetic field, 10 11 par's ultra-high pressure and temperature...

Claims

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Application Information

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IPC IPC(8): G02B21/14
Inventor 陈建文高鸿奕李儒新徐至展
Owner SHANGHAI INST OF OPTICS & FINE MECHANICS CHINESE ACAD OF SCI
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