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Method for covalent grafting heparin on surface of polymer film

A polymer film, surface covalent technology, applied in the field of biomedical engineering, to achieve the effect of simple grafting method

Inactive Publication Date: 2005-01-12
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

α, ω-aminopropyl-polyethylene glycol (Jeffamine  ED) is similar to the molecular structure of PEG, and also has good hydrophilicity and biocompatibility, but there are few reports on the use of free terminal amino groups in its structure to immobilize heparin molecules on the surface of materials.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] PP film (6×4cm 2 ) was extracted with absolute ethanol on a Soxhlet extractor for 4 hours, and then ultrasonically cleaned for 10 minutes. Then put it into 10% APS aqueous solution, pass N 2 , activated at 70°C for 100min, took it out and cleaned it to remove the APS and its decomposition products attached to the surface, and according to the method shown in step (3), the surface [OOH] was measured to be 4.67×10 -7 mol / cm 2 ; Place this film in 10% (v / v) acrylic acid aqueous solution, pass N 2 20min to remove O from the solution 2 , add 1ml 15mM Mohr’s salt aqueous solution, N 2 Purge, react at 30°C for 8h, take in 0.1wt% Triton  Ultrasonic cleaning in X-100 aqueous solution was performed for 3 × 15min to remove physically adsorbed polyacrylic acid homopolymer, and the surface [COOH] measured by the method shown in step (5) was 1.12 × 10 -7 mol / cm 2 ;Add 1.5ml 0.1M Jeffamine to 20ml pH=4.5MES solution  ED600, use 0.1M HCl and NaOH solutions to adjust the pH o...

Embodiment 2

[0048] PP film (6×4cm 2 ) was extracted with absolute ethanol on a Soxhlet extractor for 4 hours, and then ultrasonically cleaned for 10 minutes. Then put it into 20% APS aqueous solution, pass N 2 , activated at 90°C for 80min, took it out and cleaned it to remove the APS attached to the surface and its decomposition products. According to the method shown in step (3), the surface [OOH] was measured to be 5.23×10 -7 mol / cm 2 ; Place this film in 30% (v / v) aqueous methacrylic acid solution, pass N 2 20min to remove O from the solution 2 , add 1ml 15mM Mohr’s salt aqueous solution, N 2 Purge, react at 50°C for 3h, take in 0.1wt% Triton  Ultrasonic cleaning in X-100 aqueous solution was performed for 3×15min to remove physically adsorbed polymethacrylic acid homopolymer (PMAA), and the surface [COOH] measured by the method shown in step (5) was 1.09×10 -7 mol / cm 2 ;Add 1.5ml 0.1M Jeffamine to 20ml pH=4.5MES solution  ED600, use 0.1M HCl and NaOH solution to adjust the...

Embodiment 3

[0050]PP film (6×4cm 2 ) was extracted with absolute ethanol on a Soxhlet extractor for 4 hours, and then ultrasonically cleaned for 10 minutes. Then put it into 5% APS aqueous solution, pass N 2 , activated at 80°C for 150min, took it out and cleaned it to remove the APS attached to the surface and its decomposition products, and according to the method shown in step (3), the surface [OOH] was measured to be 5.54×10 -7 mol / cm 2 ; Place this film in 20% (v / v) acrylic acid aqueous solution, pass N 2 20min to remove O from the solution 2 , add 1ml 15mM Mohr’s salt aqueous solution, N 2 Purge, react at 20°C for 12h, take in 0.1wt% Triton  Ultrasonic cleaning in X-100 aqueous solution was performed for 3 × 15min to remove physically adsorbed polyacrylic acid homopolymer, and the surface [COOH] measured by the method shown in step (5) was 1.21 × 10 -7 mol / cm 2 Add 1.5ml 0.1M Jeffamine to 20ml pH=4.5MES solution  ED600, use 0.1M HCl and NaOH solutions to adjust the pH of ...

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PUM

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Abstract

The method is featured as washing and extracting polymer film by alcohol, placing it in ammonium persulfate aqueous solution for forming peroxide perssad with activation initiating polymerization of vinyl monomer containing carboxy on surface by ferrisulphas ammonium, converting carboxyl to be end azyl by using 1-ethyl-3-(3-dimethyl propyl) EDC as cross-linking agent to react with a,W-aminopropyl-polyglycol and fixing heparin on polymer film surface by covalent bond through condensation of carboxyl and azyl in heparin structure.

Description

technical field [0001] The invention relates to a method for grafting heparin on the surface of a polymer film through a covalent bond to improve anticoagulant performance, and belongs to the field of biomedical engineering. technical background [0002] Heparin is an anionic mucopolysaccharide. The characteristic pentasaccharide unit in its structure can effectively complex and improve the activity of antithrombin III (antithrombin III) in the blood to form an inactive product combined with prothrombin (thrombin). , and then prevent the conversion and deposition of fibrinogen (fibrinogen) to fibrin (fibril) controlled by thrombin, so it has good anticoagulant properties [Kwon OH, Nho YC et al.Biocompatible surfaces by immobilization of heparin on diamond- like carbon films deposited on various substrates. Surf. Interface Anal., 2000, 29: 386]. [0003] Among many synthetic materials, polyethylene (PE), polypropylene (PP) or polyethylene terephthalate (PET) is easy to form ...

Claims

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Application Information

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IPC IPC(8): C08J7/16C08L5/10
Inventor 张勇冯庆玲崔福斋
Owner TSINGHUA UNIV
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