Method for covalent grafting heparin on surface of polymer film
A polymer film, surface covalent technology, applied in the field of biomedical engineering, to achieve the effect of simple grafting method
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Embodiment 1
[0046] PP film (6×4cm 2 ) was extracted with absolute ethanol on a Soxhlet extractor for 4 hours, and then ultrasonically cleaned for 10 minutes. Then put it into 10% APS aqueous solution, pass N 2 , activated at 70°C for 100min, took it out and cleaned it to remove the APS and its decomposition products attached to the surface, and according to the method shown in step (3), the surface [OOH] was measured to be 4.67×10 -7 mol / cm 2 ; Place this film in 10% (v / v) acrylic acid aqueous solution, pass N 2 20min to remove O from the solution 2 , add 1ml 15mM Mohr’s salt aqueous solution, N 2 Purge, react at 30°C for 8h, take in 0.1wt% Triton Ultrasonic cleaning in X-100 aqueous solution was performed for 3 × 15min to remove physically adsorbed polyacrylic acid homopolymer, and the surface [COOH] measured by the method shown in step (5) was 1.12 × 10 -7 mol / cm 2 ;Add 1.5ml 0.1M Jeffamine to 20ml pH=4.5MES solution ED600, use 0.1M HCl and NaOH solutions to adjust the pH o...
Embodiment 2
[0048] PP film (6×4cm 2 ) was extracted with absolute ethanol on a Soxhlet extractor for 4 hours, and then ultrasonically cleaned for 10 minutes. Then put it into 20% APS aqueous solution, pass N 2 , activated at 90°C for 80min, took it out and cleaned it to remove the APS attached to the surface and its decomposition products. According to the method shown in step (3), the surface [OOH] was measured to be 5.23×10 -7 mol / cm 2 ; Place this film in 30% (v / v) aqueous methacrylic acid solution, pass N 2 20min to remove O from the solution 2 , add 1ml 15mM Mohr’s salt aqueous solution, N 2 Purge, react at 50°C for 3h, take in 0.1wt% Triton Ultrasonic cleaning in X-100 aqueous solution was performed for 3×15min to remove physically adsorbed polymethacrylic acid homopolymer (PMAA), and the surface [COOH] measured by the method shown in step (5) was 1.09×10 -7 mol / cm 2 ;Add 1.5ml 0.1M Jeffamine to 20ml pH=4.5MES solution ED600, use 0.1M HCl and NaOH solution to adjust the...
Embodiment 3
[0050]PP film (6×4cm 2 ) was extracted with absolute ethanol on a Soxhlet extractor for 4 hours, and then ultrasonically cleaned for 10 minutes. Then put it into 5% APS aqueous solution, pass N 2 , activated at 80°C for 150min, took it out and cleaned it to remove the APS attached to the surface and its decomposition products, and according to the method shown in step (3), the surface [OOH] was measured to be 5.54×10 -7 mol / cm 2 ; Place this film in 20% (v / v) acrylic acid aqueous solution, pass N 2 20min to remove O from the solution 2 , add 1ml 15mM Mohr’s salt aqueous solution, N 2 Purge, react at 20°C for 12h, take in 0.1wt% Triton Ultrasonic cleaning in X-100 aqueous solution was performed for 3 × 15min to remove physically adsorbed polyacrylic acid homopolymer, and the surface [COOH] measured by the method shown in step (5) was 1.21 × 10 -7 mol / cm 2 Add 1.5ml 0.1M Jeffamine to 20ml pH=4.5MES solution ED600, use 0.1M HCl and NaOH solutions to adjust the pH of ...
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