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Method for increasing output of trans-11 oleic acid from rumen

A technology of trans-11 and oleic acid, applied in animal feed, additional food elements, animal feed, etc., can solve problems such as increasing the production of rumen trans-11 oleic acid

Active Publication Date: 2005-01-05
CHUANXI BEIJING DAIRY FOOD ENG TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no public report in this field in China, and there are no patents related to increasing the production of rumen trans-11 oleic acid at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] Implementation example 1 (in vitro fermentation of rumen microorganisms)

[0090] After drying and pulverizing the fine and coarse feed raw materials at 60°C, mix them evenly according to the ratio specified in this manual.

[0091] Weigh 0.5g of mixed feed, add it into a culture bottle with a volume of 100ml, add 95ml of liquid medium and 5ml of rumen inoculum, cover with a rubber stopper under the condition of CO2, and seal it with a manual aluminum cap sealing machine, then place it in the Incubate at 39°C for 60h.

[0092] Liquid medium preparation procedure is: according to 2g tryptone, 1g yeast extract powder, 1.5g glucose, 1g cellobiose, 40ml mineral solution I (0.6% K2HPO4), 40ml mineral solution II, 1ml resazurin solution (1 %) and 920ml of distilled water Add the above materials into a stainless steel pot with a cover, boil and cool under the condition of feeding CO2, then add 4g Na2CO3, 0.5g cysteine ​​hydrochloride under the condition of feeding weaker CO2 ...

Embodiment 2

[0094] Implementation example 2 (artificial rumen continuous fermentation simulation)

[0095] After drying and pulverizing the fine and rough feed raw materials at 60°C, mix them evenly according to the ratio specified in this manual, and pack them according to the standard of 20g each.

[0096] Choose a well-sealed artificial rumen fermentation tank with a volume of 2L, fill it with 800ml of buffer solution, turn on the water bath temperature control system, and after 12 hours in a constant temperature water bath at 39°C, add 800ml of rumen inoculum filtered through two layers of gauze and a certain amount of rumen contents, and start Stirring system and buffer solution peristaltic perfusion system, put 20g of feed immediately, and then feed once every 12h, and the expected effect will be achieved after 5 days. Pass CO2 within 20 minutes after each feeding.

[0097] The rumen content for inoculation is the residue of the roughage specified in the instructions after rumen cult...

Embodiment 3

[0100] Implementation example 3 (cows)

[0101] Prepare the diet according to the requirements of this manual.

[0102] The Holstein dairy cows with a milking period of 50-200 days are selected as the implementation objects.

[0103] Through the process of refueling in 7-10 days, gradually replace the original diet with the diet of the present invention.

[0104] Ten days after the replacement of the diet, the content of trans-11 oleic acid in the rumen reached the expected target.

[0105] Application of the present invention in animals will pay attention to:

[0106] 1) Regardless of whether you choose beef cattle, dairy cows or mutton sheep as the implementation object, you must ensure that the amount and ratio of feed intake can be accurately controlled, preferably under house feeding conditions;

[0107] 2) Appropriate equipment must be provided to ensure that vegetable oil can be evenly added to the supplementary concentrate feed, and the storage time of the prepared ...

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PUM

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Abstract

A process for increasing the cutput of trans-11 oleic acid in rumen features that the raw materials of conventional feed are carefully chosen, and the vegetative oil and the regulator for decreasing the negative effect of vegetative oil to digestion of rumen are used as part of feed.

Description

(1) Technical field [0001] The invention relates to improving the production of trans-11 oleic acid, an intermediate product of hydrogenation of octadecyl-carbon unsaturated fatty acids in the rumen, through dietary preparation and rumen fermentation regulation, and belongs to the technology of increasing the supply of absorbable conjugated linoleic acid precursors in the small intestine of ruminants field. (2) Background technology [0002] Medical research in the past 10 years has shown that Conjugated Linoleic Acid (CLA) has many biological functions beneficial to human health, such as anti-cancer, enhancing immunity, preventing cardiovascular disease and diabetes, while beef, mutton and Foods derived from ruminants such as milk are the only source of CLA for humans, but the average CLA content of common ruminant meat and milk products is only about 4mg / g, which is still far behind the content that can significantly promote human health. [0003] Existing published repor...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/22A23K10/30A23K10/37A23K20/158A23K20/20A23K20/22A23K20/24A23K20/26A23K50/10
CPCY02P60/87
Inventor 王加启魏宏阳周凌云黄庆生
Owner CHUANXI BEIJING DAIRY FOOD ENG TECH DEV
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