Method for preparing and preserving homogeneous bone tissue with biological activity

A technology of biological activity and preservation method, which is applied in the field of preparation and preservation of the same type of bone tissue with biological activity, can solve the problems of disappearance of biologically induced activity, decrease of biomechanical strength, and restriction of clinical application, so as to reduce the incidence of complications , improve joint function, increase the effect of healing ability

Inactive Publication Date: 2004-09-15
NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a result, the bioinductive activity of the same bone tissue after preparation disappears, the biomechanical strength decreases, and complications such as fractures, bone nonunion, and joint dysfunction occur after transplantation, which seriously restricts clinical application.
In the study of cryopreservation of free cells, cryobiologists found that slow cooling and rapid rewarming are beneficial to the survival of cells. In Europe and the United States and other countries, applied basic research on organs including heart, kidney and liver, etc., in bone tissue There is no report

Method used

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  • Method for preparing and preserving homogeneous bone tissue with biological activity

Examples

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Comparison scheme
Effect test

Embodiment 1

[0010] Example 1. In conjunction with the accompanying drawings, a preparation and preservation method of the same type of bone tissue with biological activity, the specific implementation process is: obtain the same type of bone and joint tissue under sterile conditions, remove the soft tissue and periosteum, remove the bone marrow tissue, and retain part of the joint The attachment points of capsule, ligament and muscle were soaked in chloroform-methanol, washed with normal saline and packaged. The pretreated bone tissue was placed in a programmed cooling device, and the temperature was gradually lowered to -40°C at a rate of 2°C / min for 80 minutes. The cooled bone tissue was put into a -80°C box for storage.

[0011] The mechanical data of the same kind of bone tissue prepared and preserved by the present invention for one month are as follows:

[0012] Tensile modulus Compressive modulus Tension Compression

[0013] deal with

[0014] ...

Embodiment 2

[0022] Example 2. In conjunction with the accompanying drawings, a preparation and preservation method of the same type of bone tissue with biological activity is used for limb salvage treatment for repairing and reconstructing large bone defects after resection of bone tumors.

[0023] Patient, male, 16 years old. Pain in the lower part of the right thigh for one month, confirmed by imaging and pathological examination as osteosarcoma of the lower segment of the right femur, surgically resected the tumor bone joint of the lower segment of the right femur, and the length of the resection was 16 cm. Methods The femoral allogeneic lower segment was prepared for bone reconstruction, which not only completely resected the tumor tissue, but also preserved the function of the affected limb. The use of allogeneic bone transplantation is one of the main methods of limb salvage treatment for limb tumors. According to foreign literature statistics, more than 90% of limb malignant bone ...

Embodiment 3

[0026] Example 3. In conjunction with the accompanying drawings, a preparation and preservation method of the same type of bone tissue with biological activity is used for the repair and reconstruction of bone-infected bone defects. Bone defect caused by osteomyelitis is a common infectious disease in clinical practice. Autologous bone transplantation is a commonly used treatment method. On the one hand, it brings new pain to patients. Tissue transplantation is the new treatment. The specific implementation process is as follows: obtain the same bone and joint tissue under sterile conditions, remove the soft tissue and periosteum, remove the bone marrow tissue, retain part of the joint capsule, ligament and muscle attachment points, soak in chloroform methanol, rinse with normal saline, and pack , the pretreated bone tissue was placed in a programmed cooling apparatus, and the temperature was gradually lowered to -40°C at a rate of 1.5°C / min for 60 minutes, and the cooled bon...

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Abstract

The invention relates to a method to prepare and preserve homogeneous bioactive bone tissue, its steps: 1, obtaining: on the aseptic condition, obtaining homogeneous bone tissue; 2, finishing: eliminating soft tissue and periosteum, eliminating bone marrow tissue, and reserving partial joint bursa, ligament and adhesive point of muscle; 3, defatting: dipping in chloroformic methanol, washing with physiological saline and then packaging; 4, gradient temperature reducing: placing the preprocessed bone tissue in program temperature reducer to make gradient temperature reduction up to -40 deg.C--60 deg.C for 20-80 min.; 5, preserving: putting the bone tissue in the -60 deg.C - 80 deg. C refrigerator. It can keep the integrality of bone ultramicro structure, bone inducing activity, bioforce intensity and maintain a certain quantity of survival soft bone cells.

Description

1. Technical field [0001] The invention relates to a biological material for repairing and reconstructing bone defects, in particular to a method for preparing and preserving the same type of bone tissue with biological activity. 2. Background technology [0002] The same bone tissue has the advantages of wide source, biological healing and low treatment cost, so it is widely used in the repair and reconstruction of bone and joint defects caused by various reasons. In Europe and the United States, about 1 million pieces are used every year. The clinical application of allogeneic bone transplantation in my country began in the 1950s, and a formal bone tissue bank was established in the past 10 years, and about 20,000 pieces are used every year. [0003] The products currently provided by the bone tissue bank include fresh frozen bone, freeze-dried bone and demineralized bone. Preparation process: material collection, trimming, degreasing / decalcification, sterilization, freez...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02A61F2/28A61L27/54
Inventor 施鑫吴苏稼赵建宁周光新陆盟
Owner NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A
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