Stability indicator

Abstract

The present invention relates to an indicator of a kind of bio-chemical reagents which uses peroxidase as indication system. By 1000 ml of reagent the composition of said indicator contains MOPSO 7.21g, MOPSO sodium salt 4.45g, sodium azide 0.2-1.0g, bovine serum albumin 0.2g, Qulatong-1002 5g, 4-aminoantipyrene 1.0-3.0g, peroxidase 10-30 ku and phenol-formic acid, 1.0-4.0g. By adding high-concentration sodium azide as [O] removing agent the formation speed of red quinoinmine chromopexis can be greatly delayed, the storage time of the reagent can be greatly prolonged. After one year of storage at 2-8 deg.C, the blank absorbancy of the reagent is still below 0.2A, its stabilizing time is above one year.

Description

technical field [0001] The invention is an indicator of a class of biochemical reagents using peroxidase as an indicator system in medical laboratory science. Background technique [0002] There is a class of reagents in biochemical reagents, which will produce H after a series of reactions 2 o 2 , then relying on H 2 o 2 +4-aminoaminotipyrine + chromogen (colorless) → quinone imine pigment (red) + H 2 O reacts to indicate H 2 o 2 concentration, so this reaction is called an indicator of this type of reagent. However, in liquid single reagents, the chromogen is easily converted into red quinoneimine pigment spontaneously, which shortens the storage stability period of the reagent indicator and makes the whole reagent lose its economic value. Therefore, effectively delaying the transition speed of the reagent and improving the stability of the indicator is the key to developing the liquid single-reagent dosage form of this type of reagent. Existing indicators include ...

AI Technical Summary

Problems solved by technology

In general reagents, 4-aminoaminotipyrine and chromogen (colorless) can be stabilized in different solutions. If these two chemical substances are placed in the same solution, it is easy to spontaneously form a red quinoneimine pigment. As a result, the blank absorbance of the reagent increases and the stable period of the reagent shortens

The main reason for this spontaneous change is that the oxygen in the air enters the solution, a part of which exists in the solution in the form of [O], and in the presence of peroxidase, it reacts with 4-aminoaminotipyrine and the chromogen ( Colorless) forms a reaction loop to produce a red quinoneimine pigment, resulting in an increase in the absorbance of the reagent blank