Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Methods of treating antibody-mediated pathologies using agents which inhibit CD 21

An antibody-mediated technology, applied in the direction of antibodies, pharmaceutical formulations, chemical instruments and methods, etc., can solve the problems of limited research on the role of CD21

Inactive Publication Date: 2004-07-07
LA JOLLA PHARMA
View PDF35 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0019] Although these studies investigated the involvement of the B cell activating receptors CD40 and CD152 in a mouse model of SLE, there is limited research on the role of CD21 in the ongoing immune response

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Methods of treating antibody-mediated pathologies using agents which inhibit CD 21
  • Methods of treating antibody-mediated pathologies using agents which inhibit CD 21
  • Methods of treating antibody-mediated pathologies using agents which inhibit CD 21

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0146] Example 1 Use of sCD21 to reduce the level of anti-dsDNA antibodies

[0147] C57 / B6 mice were sensitized with a model T cell-dependent antigen, which was coupled to (CA) at a ratio of 3.8 moles of oligonucleotide / mole of KLH 10 -(TG) 10 Composed of 20-mer double-stranded oligonucleotides on the keyhole: hemocyanin (KLH) (ON-KLH).

[0148] Mice were bled and subsequently administered intraperitoneally with 50 μg of alum-precipitated ON-KLH together with 2 × 10 9 A sacrificed Bordetella pertussis (B.Pertussis) organism was immunized. Fourteen days later, the mice were boosted with intraperitoneal administration of 10 μg ON-KLH. At the time of booster immunization, mice received 300 μg soluble CD21 (sCD21; Hebell et al. (1991) Science 254: 102) intravenously and 300 μg sCD21 intraperitoneally. Control mice received PBS. For the next 3 days, mice received 300 μg of sCD21 or PBS intravenously per day.

[0149] Mice were bled 7 days after the boost and IgG levels specif...

Embodiment 2

[0152] Example 2 Use of anti-CD21 antibody to reduce the level of anti-dsDNA antibody

[0153] BALB / c mice were sensitized with a model T cell-dependent antigen, which was coupled to (CA) at a ratio of 3.8 moles of oligonucleotide / mole of KLH 10 -(TG) 10 Composed of 20-mer double-stranded oligonucleotides on the keyhole: hemocyanin (KLH) (ON-KLH).

[0154] Table 2

[0155] The post / pre antibody level ratio decreased by 45% in the antibody treated group compared to the PBS treated group. Example 3A Use of anti-CD21 antibody to reduce the level of anti-dsDNA antibody in a spontaneous model of lupus

Embodiment 3A

[0155] The post / pre antibody level ratio decreased by 45% in the antibody treated group compared to the PBS treated group. Example 3A Use of anti-CD21 antibody to reduce the level of anti-dsDNA antibody in a spontaneous model of lupus

[0156] NZB / WF1(H-2 d / z ) mouse strains provide an autoimmune model to study the complex mechanisms that control the onset of autoimmunity in lupus. The animals were F1 cross progeny of NZB / B1NJ female and NZW / LacJ male mice, and they were obtained from Jackson Laboratories (Bar Harbor, Maine). The mice developed a chronic inflammatory disease of unknown origin that could be strongly influenced by genetic factors. Autoimmunity reflected by high levels of circulating anti-nuclear antibodies (including anti-double-stranded DNA antibodies); autoantibodies against RNA-protein complexes (such as RNP and Sm), ssDNA, histones, chromatin, erythrocytes, and cardiolipin; Immune complex formation and deposition in the kidney leading to proteinuria; and ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention provides methods of treating antibody-mediated pathologies, such as autoimmune diseases (eg, SLE, ITP, and thyroiditis), by ameliorating one or more symptoms of the antibody-mediated pathology using agents that inhibit CD21 / C3d interaction. The invention also provides methods for delaying the development of antibody-mediated lesions using agents that inhibit CD21 / C3d interaction.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to US Provisional Application Serial No. 60 / 292,132 (filed May 17, 2001), which is hereby incorporated by reference in its entirety. technical field [0003] The present invention relates to the field of antibody-mediated pathologies such as autoimmune diseases. More specifically, the invention relates to methods of treating patients with antibody-mediated pathologies, such as autoimmune diseases, and methods of delaying the progression of antibody-mediated pathologies using agents that interfere with CD21 / C3d interaction. Background of the invention [0004] Antibody-mediated pathologies include a variety of conditions including autoimmune disease, xenograft rejection, allograft rejection, graft versus host disease, and immune responses to continuously administered therapeutic proteins. Antibody-mediated pathology is characterized by the overproduction of autoantibodies that lead to i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K39/395A61P5/16A61P37/02A61P37/06C07K14/725C07K16/28
CPCC07K16/2896A61K2039/505A61P37/00A61P37/02A61P37/06A61P5/16
Inventor M·D·林尼克M-A·坎贝尔
Owner LA JOLLA PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com