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Maka seedling reproducing method

A technology for seedlings and maca, which is applied in the field of plant bioengineering and biology to achieve the effects of excellent variety characteristics, low cost and good economic benefits

Inactive Publication Date: 2004-02-11
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report about the rapid propagation of maca tissue culture at home and abroad, and there is no patent literature in this regard

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Select the leaves of living plants with good growth, wash them repeatedly with tap water, and treat them with 70% ethanol for 20 seconds or 5% hydrogen peroxide for 3 minutes in an ultra-clean environment. After repeated washing with sterile water, use 8% hypochlorite Salt solution for 10 minutes of disinfection. After repeated rinsing with sterile water, cut into small sections of 1 cm, used as explants for inoculation culture, callus induction, differentiation of adventitious buds and rooting culture, the culture conditions were light intensity 1500LX, daily light time For 16 hours, the culture temperature was 22±2 degrees. First carry out callus induction: adopt MS improved medium (keep the trace element in MS medium, organic element remains unchanged, the content of macroelement is KNO 3 1250mg / L, NH 4 NO 3 1650mg / L, KH 2 PO 4 370mg / L, MgSO 4 ·7H 2 O 370mg / L, CaCl 2 440mg / L), add 0.7% agar, 6BA concentration is 0.5mg / L, NAA concentration is 0.5mg / L, can i...

Embodiment 2

[0017] Select the stems of living plants with good growth, wash them repeatedly with tap water, and then treat them with 70% ethanol for 10 seconds or 5% hydrogen peroxide for 5 minutes in an ultra-clean environment. After repeated washing with sterile water, use 10% hydrogen peroxide. The sodium hypochlorite solution was disinfected for 8 minutes. After repeated rinsing with sterile water, cut into small sections of 1 cm and use them as explants for inoculation and culture for callus induction and differentiation of adventitious buds and rooting culture. The time is 16 hours, and the culture temperature is 22±2 degrees. First carry out callus induction: adopt MS improved medium (keep the trace element in MS medium, organic element remains unchanged, the content of macroelement is KNO 3 2500mg / L, NH 4 NO 3 150mg / L, KH 2 PO 4 150mg / L, MgSO 4 ·7H 2 O 200mg / L, CaCl 2 110mg / L), add 1.0% agar, 6BA concentration is 0.5mg / L, NAA concentration is 0.5mg / L, just can induce th...

Embodiment 3

[0019] The plump maca seeds that will be selected are washed with tap water, and then 10mg / L of gibberellin (GA 3 ) soaking treatment, then sterilize, and the sterilized method is the same as in Example 2, insert the sterilized seeds into the MS culture medium without exogenous hormones, add 0.9% agar or 1.0% carrageenan or directly add inert solids in the liquid medium Particles such as glass beads, perlite, vermiculite. After 3 weeks, when the seedlings grow to a height of 4-5 cm, a sterile seedling is obtained. Select aseptic seedlings with good growth, cut the leaves as explants, and cut them into 1cm segments for callus induction, adventitious bud differentiation and rooting culture. The culture conditions are light intensity of 1500LX, daily light For 16 hours, the culture temperature was 24±2 degrees. First carry out callus induction: adopt MS improved medium (keep the trace element in MS medium, organic element remains unchanged, the content of macroelement is KNO 3...

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PUM

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Abstract

The present invention relates to plant biological engineering and biological technology, and is fast reproduction of Maka seedling. The reproduction of Maka seedling includes the steps of: obtainingexplant; inducing callus; differentiating adventitious bud; rooting culture; post-rooting treatment; and transplanting after hardening seedling. MS or improved MS culture medium is adopted and in different culture stages, 6-benzyl aminopurine (6BA) and naphthalene acetic acid (NAA) in different concentration and compounding ratio are used. The present invention makes it possible to provide highquality test tube seedlings for large-scale plantation of Maka as a kind of important economic crop.

Description

technical field [0001] The invention belongs to plant bioengineering and biotechnology, in particular to a plant seedling propagation method. Background technique [0002] Maca (Maca, Lepidium meyenii Walp) is a plant of the genus Lepidium in the family Cruciferae. The common name Maca, also known as Peruvian ginseng, maka, maca-maca, maino, ayak chinchira, ayak willku, etc., is native to the South American Andes at an altitude of 3500-4500 meters, and is now mainly distributed in the Puna ecological zone in central Peru and southeast Peru Department City Puno. Maca is a medicinal and edible plant. It has been eaten in South America for more than 5,800 years. It is traditionally used to strengthen the body, improve fertility, improve sexual function, anti-depression, and anti-anemia. Maca's chemical composition identification, active ingredients, etc. have been studied. At present, maca has become a research hotspot in improving fertility, improving sexual function, anti-...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 余龙江程华孙友平金文闻吴元喜朱敏
Owner HUAZHONG UNIV OF SCI & TECH
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