Liver-protecting functional food made up by using ligustrum fruit and silkworm chrysalis and its preparation method
A technology for functional food and privet seed, applied in the directions of food preparation, application, food science, etc., can solve problems such as unfavorable entry into the international market, and achieve the effects of simple and easy operation, low cost and remarkable effect.
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Embodiment 1
[0013] 3kg of dried Ligustrum lucidum was pulverized with a pulverizer, and then extracted twice with 70% ethanol under reflux, each time for 2 hours. Filter and combine the filtrates. Add H 2 SO 4 Stir until the final mass percentage concentration is 10%, and reflux for 3 hours to recover ethanol. The precipitate was washed with an appropriate amount of NaOH solution with a pH of 8 until the filtrate became neutral, then fully washed with hot water, and filtered to obtain a crude product. Decolorize with activated carbon, recrystallize twice with 95% ethanol to obtain oleanolic acid.
[0014] Wash 3 kg of fresh tussah silkworm pupae successively with tap water and deionized water, twist them into silkworm pupa pulp with a meat grinder, and put them into a hydrolysis kettle. Add 4 times the mass of water, stir evenly and heat to 65°C, then add 1% papain, keep stirring at constant temperature for 24 hours to carry out enzymatic hydrolysis. After the enzymatic hydrolysis is...
Embodiment 2
[0016] 5kg of dried Ligustrum lucidum was pulverized with a pulverizer, and then extracted 3 times with 75% ethanol under reflux, each time for 2 hours. Filter and combine the filtrates. Add H 2 SO 4 Stir until the final mass percentage concentration is 10%, and reflux for 3 hours to recover ethanol. The precipitate was washed with an appropriate amount of NaOH solution with a pH of 8 until the filtrate became neutral, then fully washed with hot water, and filtered to obtain a crude product. Decolorize with activated carbon, recrystallize twice with 95% ethanol to obtain oleanolic acid.
[0017]Wash 5 kg of fresh tussah silkworm chrysalis successively with tap water and deionized water, twist into silkworm chrysalis pulp with a meat grinder, and put it into a hydrolysis kettle. Add 4 times the mass of water, stir evenly and heat to 70°C, then add 2% papain, keep stirring at constant temperature for 24 hours to carry out enzymatic hydrolysis. After the enzymatic hydrolysis...
Embodiment 3
[0019] 5kg of dried Ligustrum lucidum was pulverized with a pulverizer, and then extracted 3 times with 75% ethanol under reflux, each time for 2 hours. Filter and combine the filtrates. Add H 2 SO 4 Stir until the final mass percentage concentration is 10%, and reflux for 3 hours to recover ethanol. The precipitate was washed with an appropriate amount of NaOH solution with a pH of 8 until the filtrate became neutral, then fully washed with hot water, and filtered to obtain a crude product. Decolorize with activated carbon, recrystallize twice with 95% ethanol to obtain oleanolic acid.
[0020] Wash 5 kg of fresh tussah silkworm chrysalis successively with tap water and deionized water, twist into silkworm chrysalis pulp with a meat grinder, and put it into a hydrolysis kettle. Add 4 times the mass of water, stir evenly and heat to 68°C, then add 2% papain, keep stirring at constant temperature for 24 hours to carry out enzymatic hydrolysis. After the enzymatic hydrolysi...
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