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Medicine composition, preparing method thereof and its use for preparing medicines for curing SARS and SARS-induced cardio-cerebro complication

A technology of composition and medicine, which is applied in the field of pharmaceutical composition, its preparation and its application in the preparation of drugs for the treatment of SARS and heart and brain complications caused by SARS, which can solve the problem of low ganglioside content and low activity, etc. question

Inactive Publication Date: 2003-11-12
JILIN ZHENAO PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] Rabbit meat extracts, such as Xinxuetong injection, are made by hydrolysis of rabbit muscle and rabbit myocardium. Each milliliter contains less than 1 gram of rabbit muscle, and only contains active polypeptides of muscle tissue, and its activity is low.
[0016] The contents of gangliosides in the currently disclosed brain extracts are all low, and although there are also public reports of gangliosides as components of the composition, and pharmaceutical compositions that work synergistically with other components, there is still no There are related reports that it plays a synergistic role with other animal tissues

Method used

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  • Medicine composition, preparing method thereof and its use for preparing medicines for curing SARS and SARS-induced cardio-cerebro complication
  • Medicine composition, preparing method thereof and its use for preparing medicines for curing SARS and SARS-induced cardio-cerebro complication
  • Medicine composition, preparing method thereof and its use for preparing medicines for curing SARS and SARS-induced cardio-cerebro complication

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] Embodiment 1 step (1):

[0090] Take 10kg of fresh rabbit muscle, add 20L of water to homogenate, refrigerate at 4°C for 12 hours, filter with 80-mesh nylon cloth, add 0.1% mycoprotease to the filtrate, adjust the pH value to 7.4, and incubate at 45°C for 2.5 hours. The hydrolyzate was centrifuged at 3000 rpm for 20 minutes. The centrifuged supernatant was filtered through a 0.3um filter membrane, and the filtrate was concentrated under reduced pressure to 800ml, which was used as a rabbit muscle extract for future use, wherein the polypeptide content was 8mg / ml. Step (2):

[0091] Get 10kg of fresh bovine brain and homogenate with 40L acetone, filter the homogenate with filter paper to obtain 2500g of filter residue, extract the filter residue three times with 10L of chloroform-methanol (1:1) mixture, filter respectively and combine the filtrates, use a rotary evaporator, Evaporate to dryness under reduced pressure at 37°C to obtain 1.25 ...

Embodiment 2

[0096] Embodiment 2 step (1):

[0097] Take 10kg of fresh deer muscle, add 16L of water to homogenate, refrigerate at 6°C for 14 hours, filter with 80 mesh nylon cloth, add 0.05% mycoprotease to the filtrate, adjust the pH value to 7.2, and keep it warm at 39°C for 4 hours. The hydrolyzate was centrifuged at 3000 rpm for 20 minutes. The centrifuged supernatant was filtered through a 0.3um membrane filter, and the filtrate was concentrated under reduced pressure to 800ml, wherein the polypeptide content was 2.725mg / ml, which was used as deer muscle extract for future use. Step (2):

[0098] Take 10kg of fresh bovine brain and homogenate with 35L acetone, filter the homogenate with filter paper to obtain 2500g of filter residue, extract the filter residue three times with 7.5L of chloroform-methanol (5:1) mixture, filter respectively and combine the filtrates, use a rotary evaporator, Evaporate to dryness under reduced pressure at 37°C to obtain 1.2...

Embodiment 3

[0103] Embodiment 3 steps (1):

[0104] Take 10 kg of fresh rabbit muscle, add 25 L of water to homogenate, refrigerate at 8°C for 16 hours, filter with 80-mesh nylon cloth, add 0.5% mycoprotease to the filtrate, adjust the pH value to 7.6, and incubate at 42°C for 2 hours. The hydrolyzate was centrifuged at 3000 rpm for 20 minutes. The centrifuged supernatant was filtered through a 0.3um membrane filter, and the filtrate was concentrated under reduced pressure to 800ml, in which the polypeptide content was 5mg / ml, which was used as a rabbit muscle extract for future use. Step (2):

[0105] Get 10kg of fresh horse brain and homogenate with 30L acetone, filter the homogenate with filter paper to obtain 2500g of filter residue, extract the filter residue three times with 12.5L of chloroform-methanol (20:1) mixture, filter respectively and combine the filtrates, use a rotary evaporator, , and evaporated to dryness under reduced pressure at 37°C to ob...

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Abstract

The invention discloses a kind of compound medicine which contains active polypeptide extracted from muscle organism of mammal except human and ganglioside extracted from brain organism of mammal except human. The weight of polypeptide is 6.1-100 times than the sialinic acid in ganglioside. The invention also discloses the manufacturing method and the application in the production of medicine for curing SARS and the heart and brain syndrome caused by SARS. The medicine in the invention can improve metabolism of the heart and brain organism, take part in the growth, differentiation and regeneration of nerve cell in brain, improve the blood circulation of brain and metabolism function of brain, it can cure functional disorder caused by cardiac muscle and brain disease.

Description

technical field [0001] The present invention relates to a kind of pharmaceutical composition, its preparation method and its use, in particular, to a kind of active polypeptide extracted from the muscle tissue of mammals other than human and extracted from the brain tissue of mammals other than human. The pharmaceutical composition prepared from the ganglioside, its preparation method and its pharmaceutical use in the preparation and treatment of SARS and the treatment of dysfunction caused by myocardial and brain diseases. Background technique [0002] Gangliosides are sialic acid-containing glycosphingolipids, one or more of which terminal sugar groups are N-acetylneuric acid, sialic acid. The membrane lipids of gray matter contain more than 6% gangliosides, which play an important role in nerve conduction. [0003] The clinical medicinal value of gangliosides are: for the functional recovery of central nervous system structural damage caused by various reasons; for the t...

Claims

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Application Information

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IPC IPC(8): A61K35/30A61K35/34A61K38/17A61P11/00A61P31/14
Inventor 郭志华
Owner JILIN ZHENAO PHARMA CO LTD
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