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Reagent for colloidal gold chromatographic analysis of SARS coronavirus antigen

A coronavirus and colloidal gold technology, applied in the field of testing, can solve problems such as false negatives, long experimental cycles, and unsuitable rapid detection of diseases, and achieve rapid diagnosis and easy operation

Inactive Publication Date: 2003-11-05
GUANGZHOU WONDFO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] ②The test result is incorrect (false negative)
[0027] Cell culture is a kind of aseptic operation technology, which requires high experimental working environment and conditions (aseptic operation room, ultra-clean workbench, operation room, etc.), and requires a large number of instruments and equipment (incubator, centrifuge, microscope, centrifuge, etc.) Machines, etc.), requires a large number of professional experimental equipment (various culture vessels), the experimental technology is complex, requires experienced professional and technical personnel to operate, and the experimental cycle is very long (several months), not suitable for rapid detection of diseases

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] In this embodiment, a monoclonal antibody against SARS coronavirus is used as the solid phase, and the principle of double-antibody sandwich method is used to detect whether the serum contains SARS coronavirus antigen. The manufacturing method of the present embodiment is as follows: a, antibody preparation adopts a kind of SARS coronavirus monoclonal antibody. b. Preparation of antibody membrane

[0044] 1. Preparation of coating buffer: 0.05M pH9.6 sulfate buffer as the coating solution, filtered through a 0.22μ membrane, set aside at 4°C, valid for one week.

[0045] 2. Preparation of blocking solution: prepare 0.01M pH7.0 phosphate buffered saline (PBS), filter with 0.22μ membrane, store at 4°C for later use, valid for one week. Prepare blocking working solution: 2% BSA, 2% skimmed milk, 0.01M pH 7.0 PBS, filter with 0.22μ membrane, store at 4°C for later use, valid for three days.

[0046] 3. Coating film preparation: debug the film spraying machine, the spray vo...

Embodiment 3

[0070] In this embodiment, a polyclonal antibody against SARS-CoV is used as the solid phase, and the principle of the double-antibody sandwich method is used to detect whether the serum contains SARS-CoV antigen. The manufacturing method of this embodiment is basically the same as that of Embodiment 1, the differences are as follows:

[0071] 1. Using polyclonal antibody against SARS coronavirus as coating antibody.

[0072] 2. Preparation of coating film: debug the film spraying machine, the spray volume is 20 microliters / 35 cm, dilute the coating antibody with the coating buffer solution, the concentration is 20 μg / ml, the machine draws lines, and the distance between the two lines is 5mm, which should be carefully Evenly, let dry at room temperature for 20 minutes. Soak in the blocking solution at 37°C for 60 minutes, take it out and dry it at 37°C for two hours, and seal the bag for later use.

[0073] 3. Preparation of gold-labeled antibody: Use 0.1M potassium carbonat...

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Abstract

The present invention relates to one kind of reagent for colloidal gold chromatographic analysis of SARS coronavirus antigen. The reagent consists of coating film, absorbent paper, and glass fiber film coated with gold mark antibody adhered successively together. The present invention is used in colloidal gold chromatographic analysis of SARS coronavirus antigen in hospital, airport, custom, household, etc. Using it can obtain detection result in several minutes and this is favorable to prevent diffusion of epidemic situation.

Description

technical field [0001] The invention relates to testing technology, in particular to a reagent for detecting SARS coronavirus antigen by colloidal gold chromatography. Background technique [0002] Atypical pneumonia (also known as Severe Acute Respiratory Syndrome, Severe Acute Respiratory Syndrome, abbreviated as SARS) has been raging around the world for nearly five months. On April 16, the World Health Organization officially announced a new type of coronavirus that has never been found in humans before. It is the pathogen of SARS. Coronavirus (Corona Virus) was discovered by Almeida et al. in 1968. It was named "coronavirus" because of its shape. Under the electron microscope, the virus envelope has spikes similar to the corona. Coronaviruses are widely classified and can infect humans, poultry and livestock, and can cause poultry infectious bronchitis, mouse hepatitis, porcine encephalomyelitis, feline infectious peritonitis, etc. Human coronaviruses can cause upper r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531G01N33/543G01N33/569G01N33/68
Inventor 王继华
Owner GUANGZHOU WONDFO BIOTECH
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