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Glutamine extracting process from fermented liquid

A technology of glutamine and a process method is applied in the field of preparation of amino acids for medicinal and health care purposes, and can solve the problems of low energy consumption and low product cost, and achieve the effects of reducing dosage, avoiding conversion problems and reducing production costs.

Inactive Publication Date: 2003-07-30
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems existing in the existing ion exchange method, the purpose of the present invention is to provide a process for extracting glutamine from fermentation broth with high yield and purity, low product cost, low energy consumption and environmental protection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The main components of glutamine fermentation broth are: glutamine 35g / l, glutamic acid 8g / l, ammonium sulfate 50g / l, ammonium chloride 10g / l, a small amount of other inorganic salts, pH 6.7, fermentation broth volume 5L .

[0043] 5g of cationic polyacrylamide was used as a flocculant, added into the fermentation broth under slow stirring, then stirred rapidly for 10 minutes, left to stand for 30 minutes, and filtered. Wash the solid phase with 500 ml deionized water. The supernatant and the washing water are combined and then ultrafiltered through a tubular membrane module. The molecular weight cut off of the ultrafiltration membrane is 6000. Adjust the pH of the fermented liquid to 6 with 2M sulfuric acid, and perform electrodialysis. The electrodialyzer consists of two positive membranes and one negative membrane. During this process, use 2M sulfuric acid and 2M sodium hydroxide to control the pH of the fermentation broth to stabilize at about 6, and control the t...

Embodiment 2

[0045] The main components of glutamine fermentation broth are: glutamine 32g / l, glutamic acid 7g / l, ammonium sulfate 50g / l, ammonium chloride 6g / l, a small amount of other inorganic salts, pH 6.6, volume of fermentation broth 5L .

[0046] 6g of chitosan was used as flocculant, added into the fermentation broth under slow stirring, then stirred rapidly for 15 minutes, left to stand for 30 minutes, and filtered. Wash the solid phase with 500 ml deionized water. The supernatant and the washing water are combined and ultrafiltered through a flat-panel membrane ultrafiltration device, and the molecular weight cut-off of the ultrafiltration membrane is 10,000. Use 2M hydrochloric acid to adjust the pH of the fermentation broth to 5, and perform electrodialysis. The electrodialyzer consists of two positive membranes and one negative membrane. During this process, use 2M hydrochloric acid and 20% ammonia water to control the pH of the fermentation broth to be stable at about 5, an...

Embodiment 3

[0048] The main components of glutamine fermentation broth are: glutamine 38g / l, glutamic acid 10g / l, ammonium sulfate 40g / l, ammonium chloride 20g / l, a small amount of other inorganic salts, pH6.7, fermentation broth volume 5L .

[0049] 7g of non-ionic polyacrylamide was used as a flocculant, added to the fermentation broth under slow stirring, then stirred rapidly for 15 minutes, left to stand for 30 minutes, and filtered. Wash the solid phase with 500 ml deionized water. The supernatant and the washing water are combined and ultrafiltered through a hollow fiber membrane device, and the molecular weight cut-off of the ultrafiltration membrane is 30,000. Use 2M nitric acid to adjust the pH of the fermentation broth to 6.5, and perform electrodialysis. The electrodialyzer consists of two positive membranes and one negative membrane. In this process, 2M sulfuric acid and 2M sodium hydroxide are used to control the pH of the fermentation broth to be stable at about 6.5, and t...

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PUM

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Abstract

The present invention belongs to the field of medicine and health care article amino acid, and includes pre-treatment of the fermented liquid to eliminate thallus, partial protein, pigment and polysaccharide, controlling the pH value of the fermented liquid after pre-treatment; electrodialysis to eliminate inorganic salt and regulating pH value again; leading in processed OH type anionic exchangecolumn to adsorb impurities and separate glutamine; decoloring, vacuum concentration, isoelectric crystallization, washing coarse crystal in organic solvent and drying. The said process has low exchange resin consumption, no conversion of glutamine in strong acid and strong alkali environment, low production cost and high total glutamine extracting rate up to 70%, and is suitable for industrial production.

Description

technical field [0001] The invention relates to a process for extracting glutamine from fermentation liquid, belonging to the technical field of amino acid preparation for medicine and health care. Background technique [0002] L-glutamine (Gln) is the γ-carboxyl amidate of L-glutamic acid (Glu), and is one of the 20 basic amino acids that constitute proteins. Its chemical formula is H 2 NCO·CH 2 ·CH 2 ·CH(NH 2 )·COOH, with a molecular weight of 146.15, a decomposition temperature of 184-185°C, and an isoelectric point of 5.65, is a neutral amino acid. In recent years, medical discoveries have shown that glutamine is a conditionally essential amino acid, which plays a pivotal role in biological metabolism, and a variety of diseases will be caused when it is lacking. [0003] The main producers of glutamine by fermentation are Japan and South Korea. It is estimated that the annual output of glutamine in the world has reached about 4,000 tons, and it is showing an increa...

Claims

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Application Information

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IPC IPC(8): C07C231/24C07C237/06
Inventor 邢新会刘元帅沈金玉余立新曹竹安
Owner TSINGHUA UNIV
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