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Acetyl mannan and its preparing method and use

A technology of acetyl mannan and mannan, which is applied in the field of acetyl mannan, can solve the problems that the acetylation process of plant mannan has not been reported, so as to reduce delayed hypersensitivity and promote lymphocyte proliferation , the effect of enhancing immune function

Inactive Publication Date: 2003-06-25
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the reports on the acetylation of polysaccharides are mainly for starch and cellulose compounds, but the acetylation process of plant mannan has not been reported yet.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1, prepares mannan from the plant gum of safflower

[0023] Use a certain amount of ethanol to disperse the vegetable gum, and dissolve it with an appropriate amount of 0.02M phosphate buffer with a pH of 8.0. The final concentration of the gum is about 5%, and heat it to 60°C; use the same buffer for an appropriate amount of β-mannanase preparation Dissolved, mixed with squash gum in an amount of 5 units / g substrate, and allowed the enzyme reaction to continue for 30 minutes. The reaction mixture was autoclaved at 120°C for 10 minutes, cooled to 4°C, and centrifuged at 8,000 rpm for 15 minutes. The supernatant was added with 95% ethanol to a concentration of 40% (v / v), allowed to stand for 8 hours to precipitate, and , centrifuge at 6000 rpm for 20 minutes, remove the supernatant, take the precipitate and dissolve it again in 20ml deionized water at room temperature, use the Sevag method to deproteinize 3 times, then use 3 times 95% ethanol to precipitate fo...

Embodiment 2

[0024] Embodiment 2, the acetylation of mannan

[0025] Get 2 grams of mannan prepared in Example 1, add it into a beaker containing 250 milliliters of dimethyl sulfoxide in a water bath at 55° C. while stirring, keep it warm for 20 minutes, and add solid potassium borate to a final concentration of 0.8% (W / V ), stirred for 10 minutes, added 200 milliliters of acetic anhydride preheated at 60°C to the above solution, stirred for 5 minutes, and the product after the reaction was dialyzed in running water for 4 days until the smell of dimethyl sulfoxide and acetic anhydride disappeared completely. It was then dialyzed against distilled water at 4°C for 24 hours. The dialysate was concentrated to about 50 ml with a rotary evaporator, and powdered acetylmannan sample 1 was obtained after freeze-drying.

Embodiment 3

[0026] Embodiment 3, the determination of acetyl mannan sample 1 acetyl value

[0027] 2M hydroxylamine chloride reagent and 3.5M NaOH solution were mixed in equal volumes and stored at room temperature for 3 hours, and 2ml was mixed with 1ml acetylmannan sample 1 solution (concentration 2mg / ml) and a series of standard samples (acetylcholine of different concentrations dissolved in sodium acetate solution) mixed, heated at 100°C for 15min, cooled to room temperature and added 1ml 4.1M HCl and 1ml 0.37M FeCl 3 Solution, mixed evenly to react, after the reaction, measure the absorbance value of the solution at 540nm with a spectrophotometer (1ml water reacts with the same reagent as a contrast), draw a standard curve according to the results, and calculate the acetyl mannan sample 1 acetyl The number of mannose groups is 95% of the total number of mannose groups.

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Abstract

Acetyl mannan has molecular weight of 5,000-200,000 and its molecular skeleton is mannopyranose connected by beta-D-(1-4) glucosidic bond. In its structure, there are acetyl groups led to C2, C3 and C6 hydroxy positions of carbon cycle and in the amount of 40-95% manno groups. Plant gelatin of average molecular weight over 200,000 is reacted in the catalysis of proper amount of beta-mannase, and the high-purity mannan is purified through ethanol precipitation and other process and acetylated with acetic acid, acetate anhydride anjd other reagent. Acetyl mannan is one kind of efficient immunopetentiator with the functions of inhibiting tumor growth, promoting wound heal, resisting infection, etc. and no toxic side effect. It may be mass prepared through acetylating plant mannan.

Description

technical field [0001] The present invention relates to a kind of acetyl mannan. [0002] The present invention also relates to the preparation method of acetyl mannan. [0003] The invention also relates to the application of acetyl manna in medicine. Background technique [0004] Vegetable seed gums such as kale gum, locust bean gum and guar gum are all mannans, which are important thickening polysaccharides and are widely used in the food industry and other industries. Their main components are galactose and mannose, the main chain is composed of β-D-mannopyranose connected by 1,4-glycosidic bonds, and the α-D-galactopyranose side chain is connected at the C6 position. These mannans have relatively large molecular weights, among which the molecular weight of squash gum is generally about 1 million, the molecular weight of locust bean gum is generally about 300,000, and the molecular weight of guar gum is generally about 200,000 to 300,000; these polysaccharides are gene...

Claims

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Application Information

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IPC IPC(8): A61K31/715A61P17/02C08B37/00C12P19/04
Inventor 吴襟徐桃献何秉旺谢伟陈长风
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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