Selective cross breeding method of shellfishes

A hybrid seed production and selective technology, applied in animal husbandry and other directions, to achieve the effect of enhancing stress resistance and growth potential, fast growth and high survival rate

Inactive Publication Date: 2003-03-05
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For marine shellfish, there are many studies on DNA polymorphism, which are mainly used for the study of variation and differentiation between different populations within and between species, germplasm identification and phylogenetic research, and molecular marker-assisted breeding in the prediction of heterosis and other related and selective hybridization studies have not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 (wrinkled dish abalone):

[0022] The random amplified polymorphic deoxyribonucleic acid (RAPD) technology of conventional molecular biology is used to determine the genetic distance between different geographical groups within a species, and a certain number of individuals are randomly obtained from the population with a large genetic distance as parents for hybridization, among which it is easy The obtained population is the female parent, and its operation steps are:

[0023] 1) Take populations with different natural geographical distribution areas, randomly sample and compare the genetic distance between different populations, among them, the easy-to-obtain population is the female parent, and the male parent is not easy to obtain, and the genetic distance is far from the female parent population, random sampling Sexually mature individuals, specifically 20 male Abalone wrinkled from the Iwate Sea area of ​​Japan and 150 female Abalone wrinkled from Zh...

Embodiment 2

[0052] Embodiment 2 (wrinkled dish abalone)

[0053] The difference from Example 1 is:

[0054] 1) The male broodstock came from South Korea, among them: 30 male Abalone wrinkled and 280 female Abalone wrinkled from Zhangzidao, Changhai, China; the genetic distance between the two populations was 0.01;

[0055] 2) Seed shellfish to promote ripening: Take the wrinkled pan abalone with a shell length of 6-8cm and place it in the water body at 18°C, the accumulated temperature will reach 1000°C per day, and the density will be 25 pieces / m 3 , fed with kelp and wakame macroalgae natural bait, and the daily feeding amount is: 35% of the overall body weight of the shellfish for promoting ripening;

[0056] 3) Induced labor: Dried the gonad-developed individuals and Abalone wrinkle in the shade at 18°C ​​for 1.5 hours, then put all the female individuals in a special water tank, and injected them with ultraviolet light treatment with the temperature raised to 22°C and the irradiatio...

Embodiment 3

[0060] Embodiment 3 (chlamydon scallop)

[0061] The difference from Example 1 is:

[0062] 1) The male parents are from Japan, including: 20 male Chlamys scallops and 130 female Chlamys scallops from Dalian, China; the genetic distance between the two populations is 0.07;

[0063] 2) Breeding shellfish to promote ripening: place clam shells with a shell height of 5-8cm in 18°C ​​water, the effective accumulated temperature is 160°C per day, and the density is 60 shells / m 3 , fed with natural baits such as golden algae, diatoms, chlorella, and Pyramis sp., with a feeding rate of 200,000 cells / ml per day, divided into 6 feedings, changing the water once a day, and filling with natural air to supplement oxygen Insufficient, control the light to 400Lux; when mature, the gonads are plump and shiny, the female gonads are orange-red, and the male gonads are milky white.

[0064] 3) Induced labor: dry the individuals with mature gonads in the shade at 17°C for 1 hour, then place th...

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PUM

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Abstract

The present invention relates to a selective cross breeding method of shellfish and is characterized by that it utilizes RAPD technology to define intraspecific genetic distance between different geographical colonies, and uses the individual randomly obtained from colony with larger genetic distance as parent to make hybridization, and said invented method includes the following steps: 1. seed shellfish: its selected male parent is randomly sampled pubescent individual which is not easily obtained and has larger genetic distance from female parent colony; 2. promoting maturation; using pubescent individual, using 10-20% of female shellfish application quality as application quantity of male shellfish, placing them in water with 16-21 deg.C; 3. hastening parturition; and 4. exomixis.

Description

technical field [0001] The present invention relates to shellfish seed production technology, specifically a kind of shellfish selection using molecular marker assisted breeding technology to selectively use the genetic variation accumulated in different geographical populations of shellfish respectively, and produce heterosis that can be directly applied by hybridization Sexual hybrid seed production method. Background technique [0002] Haliotis discus hannai Ino is one of the most economically important species in the family Haliotidea in my country. It is mainly distributed in the northern part of the Japanese archipelago, the Korean Peninsula, the northern part of the Yellow Sea and the Bohai Strait of China. Abalone wrinkle is dioecious, heterogeneous fertilization, mature individual gonad females are dark blue, males are milky white, and the breeding season is from July to August in the Yellow and Bohai Sea regions. The relatively concentrated sea areas in China inc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
Inventor 张国范赵洪恩
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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