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Polynary immunoassay board

An immunoassay, multi-technology, applied in measurement devices, biological tests, material inspection products, etc., can solve the problems of wasting manpower and material resources, prolonging the time of diagnosis, etc., to save the amount, shorten the time of diagnosis, save manpower and material resources. Effect

Inactive Publication Date: 2003-02-26
GENETECH BIOTECH SHANGHAI
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  • Abstract
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Problems solved by technology

[0005] Although ELISA has high sensitivity and specificity, is widely used, and has a wide variety, the commercialized ELISA kits currently in use only have one antigen or antibody coated in each well of a 96-well plate, so one kit can only detect an antigen or antibody
If it is necessary to detect several different indicators, it is necessary to repeat almost the same test steps with different kits, which wastes manpower and material resources and prolongs the time for diagnosis

Method used

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  • Polynary immunoassay board
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Embodiment Construction

[0018] Such as image 3 Simultaneous detection of multiple diseases As shown in the schematic diagram, a fluorescently labeled secondary antibody is used to detect hepatitis antibodies in serum. The spotting array is 4×4. The left picture is the antigen matrix format A1-A4 is hepatitis A antigen, B1-B4 is hepatitis B surface antigen, C1-C4 is hepatitis C antigen, D1-D4 is hepatitis D antigen; the right picture is the detection of hepatitis antibody As a result, the results showed that the hepatitis B surface antibody was positive, the hepatitis D antibody was positive, the hepatitis A antibody and the hepatitis C antibody were negative, and the intensity of the fluorescence represented the level of the antibody content.

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Abstract

The invented polynary immunoassay plate relates to an enzyme immunoassay chip reaction device which is applied widely to the medical detection, analysis and other field. The polynary immuoassay plate has several holes with antigen fixed by chemical bond to an orifice plate, the surface of the orifice plate as solid carrier is closed and the sample to be tested is added to produce hybridized reaction with the biomolecules fixed to the orifice plate. After unreacted matter is washed off, the labeled antigen is added for hybridization, and the unreacted matter is washed off again before the label signal is detected. Several indexes may be detected simultaneously. The present invention is flexible and saving in tested sample amount, and some commercial autoamtic instruments may be used.

Description

technical field [0001] The multi-component immunoassay plate of the invention relates to a chip reaction device in enzyme immunoassay, and is widely used in the fields of medical detection, analysis and the like. Background technique [0002] In 1971, Engvall and Perlmann published an article on enzyme-linked immunosorbent assay (enzyme-linked immunosorbent assay, ELISA) for the quantitative determination of IgG, which made the enzyme-labeled antibody technology used for antigen localization in 1966 develop into trace substances in liquid specimens. method of measurement. The basic principle of this method is: ① Make the antigen or antibody bind to the surface of a certain solid phase carrier and maintain its immunological activity. ②The antigen or antibody is connected with an enzyme to form an enzyme-labeled antigen or antibody, which not only retains its immune activity, but also retains the activity of the enzyme. During the determination, the test specimen (to determi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/533G01N33/543
Inventor 张涛李宾彭永济邓富桥任一萍葛海鹏
Owner GENETECH BIOTECH SHANGHAI
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