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Method for detecting pesticide-sumiewei residue

A detection method, the technology of Methiocarb, is applied in the detection of large quantities of samples and on-site monitoring, and the field of ELISA detection of pesticide Methiocarb residues. It can solve the problems that the ELISA detection method of Methiocarb has not been reported, and reaches the level of professional technology. High, save the pre-treatment process, and analyze the effect of low cost

Inactive Publication Date: 2007-04-25
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2003, Liu Fengquan, Zhang Qi, etc. took the lead in synthesizing the Methiocarb artificial antigen and applied for the patent of Methiocarb artificial antigen synthesis method in China, but Methiocarb ELISA detection method has not been reported at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Detection of Methiocarb Residues in River Water Samples

[0041] Main conditions of Methiocarb ELISA reaction system

[0042] Add 50 μl of HOM-OVA coating material 0.6 μ g / ml to each hole in the microanalysis plate, dilute the antiserum of metolcarb 20000 times as the working concentration, 1% gelatin is used as the blocker, and the methanol content in the antigen-antibody reaction matrix (PBST) is 5%, the pH value is 7.4, the ionic strength is 3.2 mol / L, 50 μl per well in the microanalysis plate.

[0043] Sample preparation to be tested:

[0044] River water sample: Measure 1L of river water, add 50μg net weight of Methiocarb to make a 50ppb liquid sample. The above samples were detected by the ELISA detection method of Methiocarb, and the operation was as follows:

[0045] 1) Coating:

[0046] HOM-OVA is used as a coating, 0.6 μg / ml, add 50 μl to each well of the microanalysis plate, refrigerate overnight at 4 ° C, pour it out, wash it with regular washi...

Embodiment 2

[0066] Example 2 Detection of Methiocarb Residues in Soil Samples

[0067] The main conditions of the Methiocarb ELISA reaction system are the same as in Example 1.

[0068] Sample preparation to be tested:

[0069] Soil sample: Weigh 1 kg of natural field soil, add Methiocarb with a net weight of 500 μg, and prepare a solid sample of 500 ppb.

[0070] The above samples were detected by the ELISA detection method of Methiocarb, and the operation was as follows:

[0071] Step 1), 2) are the same as in Example 1.

[0072] 3) Add antibody and the mixture of antibody and sample to be tested:

[0073] Weigh 0.1g of the sample to be tested, soak it in 0.2ml of methanol for about 1 hour, take 100μl and add it to 900μl of the reaction matrix to prepare the solution to be tested, dilute 10000-fold antiserum of Methiocarb with the volume of the solution to be tested and the blank matrix solution Mix well, then add 50 μl per well into the micro-analysis plate, react at 37°C for 1 hou...

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Abstract

The present invention belongs to the field of residual pesticide analysis and agricultural product safety detection. The present invention adopts the detection formula of Logit(B / Bo)=-0.9404LogC+1.5146, where B is the extinction value of the sample hole, Bo is positive contrast extinction value, and C is the metolcarb concentration determined inside the diaphragm orifice. The detection conditions include metolcarb coating antigen MOM-OVA of 0.6 microgram / ml concentration being 50 microliter in each diaphragm orifice, metolcarb antiserum diluted for 20000 times as the work concentration, 1 % concentration gelatin as closure, PBST with methanol in 5 %, pH 7.4, and ion strength of 3.2 mol / L. The present invention has high specificity, high sensitivity and high stability, and is fast, sensitive and specific micro metolcarb detecting method.

Description

(1) Technical field [0001] The invention discloses an ELISA detection method for the residual amount of the pesticide metacarb, belonging to the field of biotechnology. It is specially used for high-sensitivity and rapid detection of metolcarb pesticide residues in agricultural products and agricultural production environments, and is especially suitable for large-scale sample detection and on-site monitoring. (2) Background technology [0002] Metolcarb (metolcarb) belongs to carbamate chemical insecticides, and is one of the pesticide varieties produced in large tonnage in my country. Methiocarb preparations have moderate toxicity and are mainly used to control pests in cotton, rice, tea trees, citrus and other crops. They have the characteristics of wide insecticidal spectrum, long residual effect and high killing rate. At present, many manufacturers in China have carried out large-scale production of the original drug or its compound preparation and have been well promo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/547G01N33/543G01N33/535G01N33/52G01N21/31
Inventor 刘凤权胡白石张奇徐丽娜姜英华
Owner NANJING AGRICULTURAL UNIVERSITY
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