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Method for increasing conversion of hydrocortisone

A technology of pine conversion rate and high-pressure treatment, which is applied in the field of improving the conversion rate of hydrocortisone, can solve the problems of affecting the conversion reaction rate and yield, low solubility of steroid compounds, and low conversion rate of low pine, so as to promote development and utilization , Improve solubility and improve conversion rate

Inactive Publication Date: 2006-11-22
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the problems of poor solubility of hydrocortisone conversion substrate RSA (17α-hydroxyl-pregn-4-ene-3,20-dione-21-acetate) and low conversion rate of hydrocortisone; A New Method for Improving the Conversion Rate of Hydrocortisone
[0007] The present invention studies the effects of different pressures, processing time and oxygen carriers on the conversion rate of hydrocortisone, proposes a technical method for improving the conversion rate of hydrocortisone, and solves the problem of the low solubility of steroidal compounds in water and the influence on the conversion reaction rate And productive rate, the higher problem of production cost, thereby improved the conversion ratio of hydrocortisone

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] ·Thalline activation: under aseptic conditions, inoculate the mycelia of M. pearhead in PDA medium, culture at 30°C for 5-6 days, and store at 4°C for later use.

[0019] Preparation of spore suspension: Take 1 tube of activated slant bacteria, add 5mL sterile water, wash the spores, and prepare 1 × 10 6 ~10 7 per mL of spore suspension.

[0020] ·Blue pear head mold liquid culture: take 1mL of spore suspension and inoculate it into the liquid fermentation medium, and culture at 30°C, 200 / min, for 24h.

[0021] Steroid feeding method: mix RSA with 80% ethanol solution in a ratio of 1:16 (W / V), that is, grind 0.3 gram of steroid substrate RSA into powder, and mix with 4.8mL concentration of 80% ethanol solution, Heat and reflux in the flask to dissolve until clarification, and quickly put the 4.8mL solution into 100mL fermentation broth for conversion, that is, the RSA feed ratio is 0.3% (W / V).

[0022] Transformation reaction: adjust the pH value of the transformatio...

Embodiment 2

[0024] In this example, after the conversion liquid was treated under normal pressure for 30 hours, the pressure was increased to perform high pressure treatment for 8 hours, and finally the treatment was continued under normal pressure for 10 hours, and other conditions were the same as in Example 1. Under this condition, the conversion rate of hydrocortisone increased by 4.2% compared with the normal pressure control condition.

Embodiment 3

[0026] In this embodiment, after the conversion liquid was treated under normal pressure for 36 hours, the pressure was increased for 6 hours of high pressure treatment, and finally the treatment was continued under normal pressure for 6 hours. Others are the same as embodiment 1. Under this condition, the conversion rate of hydrocortisone was increased by 28.2% compared with normal pressure control condition.

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Abstract

The method for improving the conversion rate of hydrocortisone belongs to the method for improving the conversion rate of hydrocortisone by improving the solubility of the conversion substrate RSA and controlling the morphology of bacteria through pressurized treatment. It solves the problems of low yield and high cost in the current production of hydrocortisone. It is based on the blue pear head mold or Curvularia crescenae as the starting strain, and the seed is expanded and cultivated to obtain the bacterial culture solution, which is put into a steroid substrate. When the transformation reaction is carried out, 0~3mmol / L oxygen carrier is added. And control the pressure, that is, not only normal pressure treatment, but also high pressure treatment is required. When high pressure is applied, the pressure is 0.5-1 MPa, and the constant pressure treatment is 4-8 hours. The process of the method is simple, the conversion rate of the hydrocortisone is increased by 4.2-28.2% compared with the control condition of normal pressure, the production cost is reduced, and the further development and utilization of the product are promoted. The invention provides a new process for the production of hydrocortisone, which has both economic benefits and social benefits.

Description

technical field [0001] The invention belongs to the technology of improving the conversion rate of hydrocortisone. It particularly relates to a method for improving the solubility of the conversion substrate RSA by using high pressure, increasing the dissolved oxygen level, controlling the shape of bacteria, and increasing the conversion rate of hydrocortisone. Background technique [0002] Steroid hormone drugs are an important class of drugs in clinical application. With the continuous expansion of the application range of steroid drugs, the steroid medicine industry has shown a good development trend. From the perspective of the development of the international steroid pharmaceutical industry, the output in 1980 was about 9.5 tons, with sales of 1.5 billion US dollars, accounting for 4.3% of the total sales of pharmaceutical products; in 1990, the output increased to 105 tons, with sales of 10.8 billion US dollars, with an annual average Increased by 10.4%; sales in 2000...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P33/00C12R1/65
Inventor 贾士儒乔长晟徐旭范志华
Owner TIANJIN UNIV OF SCI & TECH
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