Method for increasing conversion of hydrocortisone
A technology of pine conversion rate and high-pressure treatment, which is applied in the field of improving the conversion rate of hydrocortisone, can solve the problems of affecting the conversion reaction rate and yield, low solubility of steroid compounds, and low conversion rate of low pine, so as to promote development and utilization , Improve solubility and improve conversion rate
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Embodiment 1
[0018] ·Thalline activation: under aseptic conditions, inoculate the mycelia of M. pearhead in PDA medium, culture at 30°C for 5-6 days, and store at 4°C for later use.
[0019] Preparation of spore suspension: Take 1 tube of activated slant bacteria, add 5mL sterile water, wash the spores, and prepare 1 × 10 6 ~10 7 per mL of spore suspension.
[0020] ·Blue pear head mold liquid culture: take 1mL of spore suspension and inoculate it into the liquid fermentation medium, and culture at 30°C, 200 / min, for 24h.
[0021] Steroid feeding method: mix RSA with 80% ethanol solution in a ratio of 1:16 (W / V), that is, grind 0.3 gram of steroid substrate RSA into powder, and mix with 4.8mL concentration of 80% ethanol solution, Heat and reflux in the flask to dissolve until clarification, and quickly put the 4.8mL solution into 100mL fermentation broth for conversion, that is, the RSA feed ratio is 0.3% (W / V).
[0022] Transformation reaction: adjust the pH value of the transformatio...
Embodiment 2
[0024] In this example, after the conversion liquid was treated under normal pressure for 30 hours, the pressure was increased to perform high pressure treatment for 8 hours, and finally the treatment was continued under normal pressure for 10 hours, and other conditions were the same as in Example 1. Under this condition, the conversion rate of hydrocortisone increased by 4.2% compared with the normal pressure control condition.
Embodiment 3
[0026] In this embodiment, after the conversion liquid was treated under normal pressure for 36 hours, the pressure was increased for 6 hours of high pressure treatment, and finally the treatment was continued under normal pressure for 6 hours. Others are the same as embodiment 1. Under this condition, the conversion rate of hydrocortisone was increased by 28.2% compared with normal pressure control condition.
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