Compositions useful as fibrin sealants

A technology for fibrin and fibrin monomers, which is applied in the field of compositions of fibrin monomers, and can solve the problems of ineffectiveness and the like

Inactive Publication Date: 2000-07-19
BRISTOL MYERS SQUIBB CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fibrinolytic factors are thought to prematurely break down the sealant clot and not have the desired effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] 120 cc of the patient's blood was drawn and mixed with 17 cc of 4% trisodium citrate USP for anticoagulation. About 60cc of plasma was separated by centrifugation, and it was reacted with biotin-thrombin at 37°C for 10 minutes by stirring gently. This biotin-thrombin catalyzes the release of fibrinopeptide A from fibrinogen only, without activating factor XIII. This forms acid-soluble fibrin I polymers. The fibrin I polymer was isolated by centrifugation and dissolved in 3.5 ml of 0.2M sodium acetate buffer (pH 4) in the presence of calcium ions. Avidin covalently bound to agarose is added to the solution, which chemically binds to biotin-thrombin, which is then separated from the fibrin I polymer by filtration: avidin-agar sugar complex. The resulting fibrin monomer solution had the composition shown in Table 1. Example 2-24

Embodiment 2-24

[0012] Twenty-three additional blood samples were processed as described in Example 1 and the resulting composition analyzed. The results were converted into the ranges shown in Table 1. Table 1 also shows the plasma levels of each component in 24 patients and further compares the formulation of the present invention with Tissucol (Immuno), Beriplast (Behringwerke) and Bolheal (Kaketsuken) blocking agents.

[0013] Fibrin Monomer Composition

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PUM

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Abstract

Novel fibrin monomer compositions which are solutions including additional coharvested components, such as prothrombin and Factor XIII, are useful in fibrin sealant applications. Preferably, the compositions are autologous to the patient receiving the sealant and these compositions may also include coharvested plasminogen, Factor X, antithrombin III and / or fibronectin.

Description

field of invention [0001] The present invention relates to compositions of fibrin monomer, and more particularly to concentrated fibrin monomer solutions comprising other blood proteins or components, wherein the fibrin monomer as well as the blood proteins or components are native. These compositions are useful, for example, as fibrin sealants. Background of the invention [0002] Edwardson et al. in US Patent No. 5,750,657 described a novel method for preparing a fibrin sealant from a fibrin monomer composition. Fibrin monomer is used in a non-dynamic form produced by pretreatment of fibrinogen with thrombin-like enzymes. When it is administered to a patient in need of treatment, this non-dynamic state is reversed so that the monomer can polymerize to form a fibrin polymer that can be used as a fibrin sealant. [0003] The stability of fibrinolysis is one of the main points of concern when using fibrin sealants. It is generally believed that fibrinolytic factors break d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/00A61K35/14A61K38/43A61K38/46A61L24/10A61P7/00
CPCA61L24/106A61P7/00A61P7/04
Inventor P·A·D·爱德华森J·E·费尔布洛特D·A·霍林斯贝S·A·塞德霍尔姆-威廉斯
Owner BRISTOL MYERS SQUIBB CO
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