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Notoginseng adventitious root tissue culture and its production method

A production method and tissue culture technology, applied in the field of tissue culture and production of Panax notoginseng adventitious root, can solve problems such as staying, achieve short growth time, solve natural condition restrictions, and achieve significant economic and social benefits

Inactive Publication Date: 2006-05-24
高文远 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the work on the tissue culture of medicinal plants in my country stays at the stage of petri dishes and shake flasks. So far, the in vitro induction and cultivation of Panax notoginseng adventitious roots and the use of bioreactor technology to cultivate in vitro induced Panax notoginseng adventitious roots have been There is still no report at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] Adjust 100mL MS solid medium to pH 6.0 with HCl and NaOH solution, at 121°C, 1.2kg / cm -2 Sterilize under high pressure for 15 minutes for later use; select Panax notoginseng roots that grow well and are free from diseases and insect pests, rinse and disinfect with tap water, distilled water and detergent for 10 minutes, then wash with distilled water, soak in 70% ethanol for 3 minutes, and then use Disinfect with 1% sodium hypochlorite (add a few drops of surfactant Tween-20) for 30 minutes, and finally wash with distilled water for 3-5 times to obtain aseptic notoginseng roots. Cut the sterilized roots of notoginseng into small pieces with a sterilized scalpel, inoculate them on the MS solid medium prepared in advance, and induce the callus of notoginseng at 25°C and in the dark, and culture them for 4 weeks Visible callus formation. Adjust 100mL MS solid medium to pH 6.0 with HCl and NaOH solution, at 121°C, 1.2kg / cm -2 Sterilize under pressure for 15 minutes and se...

Embodiment 2

[0010] Adjust 100mL 1 / 2MS solid medium to pH 6.0 with HCl and NaOH solution, at 121°C, 1.2kg / cm -2 Sterilize under high pressure for 15 minutes for later use; select Panax notoginseng roots that grow well and are free from diseases and insect pests, rinse and disinfect with tap water, distilled water and detergent for 10 minutes, then wash with distilled water, soak in 70% ethanol for 3 minutes, and then use Disinfect with 1% sodium hypochlorite (add a few drops of surfactant Tween-20) for 30 minutes, and finally wash with distilled water for 3-5 times to obtain aseptic notoginseng roots. Cut the sterilized notoginseng roots into small pieces with a sterilized scalpel, inoculate them on the 1 / 2MS solid medium prepared in advance, and induce the notoginseng callus at 24°C and dark conditions, and culture for 5 Callus formation can be seen after one week. Adjust 100mL 1 / 2MS solid medium to pH 6.0 with HCl and NaOH solution, at 121°C, 1.2kg / cm -2 Sterilize under pressure for 15...

Embodiment 3

[0012] Adjust 100mL 3 / 4MS solid medium to pH 6.0 with HCl and NaOH solution, at 121°C, 1.2kg / cm -2 Sterilize under high pressure for 15 minutes for later use; select Panax notoginseng roots that grow well and are free from diseases and insect pests, rinse and disinfect with tap water, distilled water and detergent for 10 minutes, then wash with distilled water, soak in 70% ethanol for 3 minutes, and then use Disinfect with 1% sodium hypochlorite (add a few drops of surfactant Tween-20) for 30 minutes, and finally wash with distilled water for 3-5 times to obtain aseptic notoginseng roots. Cut the sterilized notoginseng roots into small pieces with a sterilized scalpel, inoculate them on the prepared 3 / 4MS solid medium, induce the notoginseng callus at 25°C and dark conditions, and culture for 5 Callus formation can be seen after one week. Adjust 100mL 3 / 4MS solid medium to pH 6.0 with HCl and NaOH solution, at 121°C, 1.2kg / cm -2 Sterilize under pressure for 15 minutes and se...

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Abstract

The invention discloses a tissue culture and production method of notoginseng adventitious root. It includes: induction of notoginseng callus; expansion and subculture of notoginseng callus; induction of notoginseng adventitious root; propagation and subculture of notoginseng adventitious root; screening and activity of different root systems of notoginseng adventitious root Determination and multiplication of ingredients; and reactor cultivation of Radix Notoginseng adventitious root and other steps. The adventitious root of Radix Notoginseng provided by the present invention uses a reactor to cultivate the adventitious root of Radix Notoginseng, and its multiplication factor can reach more than 100 times. It is suitable for the growth of notoginseng adventitious roots, so it opens up a new way of industrialization for the large-scale production of notoginseng, a precious Chinese herbal medicine resource, which can fundamentally solve the problem that the source of notoginseng is limited by natural conditions, so it is economical and significant social benefits.

Description

technical field [0001] The invention relates to a tissue culture and production method of adventitious roots of plants, in particular to a tissue culture and production method of notoginseng adventitious roots. Background technique [0002] Panax notoginseng is one of the most commonly used Chinese medicinal materials in my country, and it is also a best-selling herbal medicine in the international market. It can be directly eaten as a tonic, and it is also an important raw material for many health products and medicines, such as Xuesaitong, Yunnan Baiyao and Danshen dripping pills. Due to the limitation of geographical conditions, the cultivation area of ​​this medicinal material is limited, and it is mainly distributed in a narrow area around Wenshan Prefecture, Yunnan Province, my country, and it is also affected by "the cultivated land cannot be reused". Since the problem of "old seven land" cannot be solved, the land for cultivating Panax notoginseng can only be expand...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 高文远贾伟
Owner 高文远
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