L-arginine producing strain and its mutation method and usage in producing L-arginine

Abstract

The present invention relates to the fermentation process of producing L-arginine. By using Corynebacterium crenatum SYA5 screened and preserved by the present lab as parent and through conventional physical and chemical mutation process and multiple structural analog resistance screening, mutant strain SDNN403 is obtained. Through culturing of the mutant strain under optimized condition, L-arginine is produced in the yield level of 30-35 g / L. The strain has high genetic stability, stable yield characteristic, high L-arginine yield level, less produced hetero acids and easy technological amplification, and is suitable four industrial production.

Description

technical field [0001] A bacterial strain producing L-arginine, its mutagenesis method and a method for producing L-arginine using the bacterial strain. The present invention relates to using glucose and ammonium sulfate as main raw materials and utilizing Corynebacterium crenatum to mutate A method for producing L-arginine by strain fermentation. Background technique [0002] L-arginine is a semi-essential amino acid for human body and animals. It has important biochemical and physiological significance, so it is widely used in food and pharmaceutical industries, especially in the field of pharmaceutical industry. Medicines for the treatment of vascular diseases and enhancing sperm motility in men have received attention. [0003] The preparation methods of L-arginine include extraction method (Chinese patent CN1161959) and fermentation method. The extraction method is time-consuming, has low yield, serious environmental pollution, and is not suitable for large-scale produ...

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Problems solved by technology

The mutagenesis methods and structural analogues used in the above method are often relatively simple, which may easily cause poor genetic stability of mutant strains with high yield of L-arginine, and produce back mutations in large-scale production, thereby causing the decline of L-arginine yield traits

Fermentative production of L-arginine, there is also the use of Escherichia coli or Corynebacterium glutamicum through genetic modification to introduce the gene of the key enzyme or increase the copy number of the key enzyme, thereby increasing the L-arginine production of the obtained recombinant bacteria (Chinese patent CN1276421 and U.S. patent US4430430), but the exogenous plasmid of the recombinant bacterial strain that above-mentioned method obtains is easy to lose in production or needs to add certain selective pressure, makes adopting this type of recombinant bacteria to produce L-arginine It is difficult to scale up the process and realize industrialization

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