Rapid detection method of melamine liquid chromatography

A melamine and liquid chromatography technology, applied in the field of melamine detection, can solve the problems of high cost, shortened melamine detection time, and long detection time, and achieve the effects of improving production efficiency, shortening production cycle, and reducing production cost

Pending Publication Date: 2022-08-09
SICHUAN NEW HOPE DAIRY
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the defects of long detection time and high cost in the detection of melamine in raw milk by the high-efficiency liquid phase method in the existing national standard, and propose a quick detection method of melamine liquid chromatography. Optimize the extraction method of melamine, so that there is no need for purification column and nitrogen blowing treatment during the detection process, and accurate, reliable and national standard melamine liquid chromatography detection results can be obtained, which significantly shortens the detection time of melamine in raw milk , and ensure the accuracy of the test results, it has a positive effect on improving production efficiency, shortening the production cycle, and reducing production costs, and is suitable for large-scale promotion and application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Weigh 5g (accurate to 0.0001g) of raw milk sample into a 50mL centrifuge tube, accurately pipette 17.5mL of 20% methanol solution and 2.5mL of 10% trichloroacetic acid solution, and mix well to obtain a mixed solution;

[0042] (2) The mixed solution was subjected to ultrasonic extraction (power 200W, frequency 40KHz, normal temperature, time 10 minutes), shaking extraction (speed 120r / min, amplitude 20mm, room temperature, shaking time 10 minutes) and centrifugal extraction (temperature 6℃) , rotating speed 10000r / min, time 10min), take the supernatant to obtain melamine extract;

[0043] (3) Detecting the melamine extract obtained by the melamine extraction method with a liquid chromatograph to obtain a chromatogram;

[0044] The chromatographic conditions are:

[0045] Chromatographic column: C18 column 250mm*4.6mm, 5μm;

[0046] Column temperature: 40℃;

[0047] Suction volume: 20μL;

[0048] VWD / DAD detector wavelength: 240nm;

[0049] Flow rate: 1.0mL / min...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a rapid detection method of melamine liquid chromatography. The rapid detection method comprises a melamine extraction method and a liquid chromatography determination method. According to the detection method, an extraction method of the melamine is optimized, so that an accurate and reliable melamine liquid chromatography detection result meeting the national standard can be obtained without purification column and nitrogen blowing treatment in the detection process, the detection time of the melamine in the raw milk is remarkably shortened, the accuracy of the detection result is ensured, and the detection efficiency is improved. The method has positive effects on improving the production efficiency, shortening the production period and reducing the production cost, and is suitable for large-scale popularization and application.

Description

technical field [0001] The invention relates to the detection field of melamine, in particular to a rapid detection method of melamine liquid chromatography. Background technique [0002] Melamine is an organic ingredient with a nitrogen content of up to 66%. Excessive intake of melamine can cause kidney stones or lead to renal failure in infants and young children, resulting in serious food safety. Therefore, in the production process of dairy products, it is necessary to measure the melamine content of raw milk to prevent unqualified dairy products with excessive melamine content from entering the market and causing damage to the health of consumers. [0003] Existing melamine detection methods include high performance liquid phase method, potentiometric titration method and gas chromatography-mass spectrometry method, etc. Among them, in the national standard, the high performance liquid phase method is adopted for the detection of melamine. However, the high-performance...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/06G01N30/02
CPCG01N30/06G01N30/02G01N2030/027G01N2030/062
Inventor 陈茂姣邓燕琴付林王峰王金玉兰宁何刚
Owner SICHUAN NEW HOPE DAIRY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap