Methods and compositions for cosmetic and cosmetic treatment and stimulation of hair growth
A technology of composition and lysate, applied in the fields of biochemical equipment and methods, microorganisms, hair care, etc., can solve the problems of reduced expression of protective proteins, damaged skin barrier, etc.
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Embodiment 1
[0252] Example 1: Culture and Production of Adherent Placental Cells
[0253] Placenta-derived cell populations containing more than 90% maternal tissue-derived cells were prepared as described in Example 1 of International Patent Application WO 2016 / 098061, which is incorporated herein by reference in its entirety.
Embodiment 2
[0255] Example 2: Culture of placental cells in serum-free medium (SFM)
[0256] method
[0257] The cell harvest and expansion process consists of 3 stages followed by downstream processing steps: Stage 1 , Intermediate Cell Stock (ICS) production; Stage 2 , thaw the ICS and proceed to the initial further incubation steps; and Stage 3 , an additional incubation step was performed in the presence of serum. Downstream processing steps include harvesting from flasks or bioreactors, cell concentration, washing, formulation, filling, and cryopreservation. This procedure involves periodic testing of the growth medium for sterility and contamination, all of which are described in International Patent Application Publication No. WO 2019 / 239295, which is incorporated herein by reference. Bone marrow migration assays were also performed as described in WO 2019 / 239295.
[0258] result
[0259] Placental cells were extracted and expanded in serum free (SF) medium for 3 passages....
Embodiment 3
[0262] Example 3: Osteocyte and Adipocyte Differentiation Assay
[0263] ASCs were prepared as described in Example 1 . BM adherent cells were obtained as described in WO2016 / 098061 by Esther Lukasiewicz Hagai and Rachel Ofir, which is hereby incorporated by reference in its entirety. Osteogenesis and adipogenesis assays were performed as described in WO 2016 / 098061.
[0264] Osteocyte induction. Incubation of BM-derived adherent cells in osteogenesis-inducing medium resulted in more than 50% differentiation of BM cells, as evidenced by positive Alizarin Red staining. In contrast, none of the placenta-derived cells showed signs of osteogenic differentiation.
[0265] Next, a modified osteogenesis medium containing vitamin D and a higher concentration of dexamethasone was used. More than 50% of BM cells underwent differentiation into osteocytes, and none of the placenta-derived cells showed signs of osteogenic differentiation.
[0266] Adipocyte induction. In adipocyte indu...
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