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Penicillium fimbriatum strain RA51 and application thereof

A technology of Penicillium vermiformis and RA51, applied in the field of microorganisms, can solve the problems of large loss of nutrients, difficulty in large-scale production, unstable detoxification effect, etc. Effect

Active Publication Date: 2022-08-02
CHINA NAT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the main disadvantages of physical and chemical methods are: the reaction conditions are too intense, the detoxification effect is unstable, the loss of nutrients is large, and it is difficult to produce on a large scale.
However, the detoxification mechanism of some microorganisms belongs to physical adsorption, not substantial biodegradation. Toxins adsorbed on the cell wall of bacteria may undergo desorption

Method used

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  • Penicillium fimbriatum strain RA51 and application thereof
  • Penicillium fimbriatum strain RA51 and application thereof
  • Penicillium fimbriatum strain RA51 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Obtainment of Penicillium filtrates strain RA51

[0050] 1. Isolation of Penicillium difficile strain RA51

[0051] Soil samples were collected from Zhoushan Beach, Zhejiang Province, and 5 g of soil samples were weighed and placed in 30 mL of sterile saline, stirred and mixed, so that the microorganisms in the soil were suspended in the water and allowed to stand for 1 h. According to the inoculum amount of 1‰, take the soil suspension and inoculate it in the liquid medium containing FB1 for the primary screening, so that the final concentration of FB1 is 500ppb. Red Bengal and streptomycin were added to the primary screening medium to inhibit the growth of actinomycetes and bacteria. Under the conditions of 30℃, 160r / min, after culturing in shake flasks for 48h, transfer to the primary screening liquid medium according to the inoculum amount of 1‰, and continue to cultivate for 48h, and transfer for three consecutive times. Finally, dilute the supernatant...

Embodiment 2

[0071] Example 2: Preliminary determination of detoxification substances of fumonisins by Penicillium fibriformis strain RA51

[0072] There are two main ways to remove fumonisins by microorganisms, one is adsorption and binding, and the other is degradation and transformation. Adsorption binding is the adsorption of toxins by the cell wall or the main components of the cell wall, such as polysaccharides and peptidoglycan, and is a reversible biological process. Degradation and transformation is a chemical reaction process in which microorganisms degrade or convert toxins through enzymatic reactions to form non-toxic or low-toxic products. Therefore, the distinction between degradation transformation and adsorption binding is the premise to understand the mechanism of fumonisin degradation by Penicillium fumonis.

[0073] 1. Cultivation of Penicillium difficile strain RA51

[0074] The Penicillium difficulum strain RA51 obtained from the initial screening was inoculated on t...

Embodiment 3

[0086] Example 3: The effect of fermentation conditions on the ability of Penicillium fibroblasts strain RA51 to degrade fumonisin FB1

[0087] 1. Degradation effect of fermentation products on FB1 at different culture temperatures

[0088] Inoculate Penicillium difficile strain RA51 in PDB medium at 28°C, 150r / min, after culturing for 7 days, the concentration of conidia obtained is 10 6 cells / mL of bacterial suspension. Under the optimal culture conditions, the bacterial suspension was inoculated into the fermentation medium at a volume ratio of 5%, and the culture temperature was set at 20°C, 25°C, 30°C, 35°C, 40°C, and 45°C, respectively. 160r / min, cultured for 6d to obtain fermentation products. Add FB1 to the fermentation product to make the final concentration 500ppb, and mix well. After co-incubating at 28°C and 160r / min for 72h, the concentration of FB1 in the fermentation products of Penicillium fugustum was detected by HPLC-MS, and the degradation rate of FB1 by ...

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Abstract

The invention discloses a penicillium fimbriatum strain RA51 and application thereof, and belongs to the technical field of microorganisms. The Penicillium delicatum strain RA51 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the Penicillium delicatum strain RA51 is CGMCC (China General Microbiological Culture Collection Center) No.23852. The Penicillium fistulosum fermentation product can be used for efficiently degrading fumonisins in a biological material. The invention also provides a liquid microbial inoculum or a solid microbial inoculum prepared by taking the penicillium fistulosum as an active ingredient, and the liquid microbial inoculum or the solid microbial inoculum is used for biodegradation and removal of fumonisins in biomass materials. The penicillium fischeri has high detoxification activity, strong specificity and mild action effect, does not have secondary pollution, can improve the quality of agricultural products and ensure the edible safety of people and livestock, and is suitable for removing fumonisins in agricultural products, feed industry and food processing.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Penicillium rubrum strain RA51 and an application thereof. Background technique [0002] According to the assessment of the United Nations Food and Agriculture Organization (FAO), about 25% of the world's crops are contaminated by mold and mycotoxins every year, and about 2% of the food is inedible due to mold contamination. Fumonisins are mainly secondary metabolites produced by Fusarium stratum, Fusarium verticillium and Fusarium florida. It is a common type of mycotoxins in food and its products, which can contaminate a variety of crops and economic crops. . The existence of fumonisins not only harms the yield and quality of crops, but also poses a serious threat to human and animal health. The toxin can cause leukomalacia in horses, pulmonary edema in pigs, and neural tube defects in infants. Epidemiological studies have confirmed that the high inciden...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A23L5/20C12R1/80
CPCC12N1/14A23L5/28
Inventor 王玲胡培松唐绍清胡时开圣忠华焦桂爱魏祥进邵高能谢黎虹
Owner CHINA NAT RICE RES INST
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