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High-efficiency composting microbial inoculum based on gradient conversion of human excrement PPLC from organic matters as well as preparation method and application of high-efficiency composting microbial inoculum

An organic matter and composting technology, applied in the field of high-efficiency composting inoculants, can solve the problems of slow speed of human excrement, large nitrogen loss, complex composition, etc., and achieve the effects of saving labor, reducing nitrogen loss, and shortening heating time.

Pending Publication Date: 2022-07-29
HARBIN INST OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of slow speed, large loss of nitrogen, complex composition and high cost of existing bacterial agents for treating human feces, the present invention provides a high-efficiency composting bacterial agent based on the stepwise conversion of organic matter into human feces PPLC, and the high-efficiency composting bacterial agent contains Mixed microbial bacterial liquid and immobilized carrier, the composition of the mixed microbial bacterial liquid is: Bacillus highlander, Bacillus halotolerant, Bacillus methylotropic, Azotobacter sphaeroides, Bacillus cereus, Candida utilis , Bacillus amyloliquefaciens

Method used

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  • High-efficiency composting microbial inoculum based on gradient conversion of human excrement PPLC from organic matters as well as preparation method and application of high-efficiency composting microbial inoculum
  • High-efficiency composting microbial inoculum based on gradient conversion of human excrement PPLC from organic matters as well as preparation method and application of high-efficiency composting microbial inoculum
  • High-efficiency composting microbial inoculum based on gradient conversion of human excrement PPLC from organic matters as well as preparation method and application of high-efficiency composting microbial inoculum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: the preparation method of high-efficiency composting bacterial agent

[0033] Step 1. Preparation of mixed microbial bacterial liquid

[0034] (1) Slope culture of strains

[0035] Bacillus highlandi, Bacillus halotolerant, Bacillus methylotrophic, Nitrogenobacter phaeotrophus, Bacillus cereus, Candida utilis, and Bacillus amyloliquefaciens in the seed preservation tube were respectively activated by slant, and then inoculated in Purification operation is carried out in a petri dish to obtain Bacillus highlandi, Bacillus halotolerant, Bacillus methylotrophic, Nitrogenococcus sphaeroides, Bacillus cereus, Candida utilis, and Bacillus amyloliquefaciens pure breeds, respectively, for use.

[0036] In step (1), the step of activating Bacillus highlandi on the slant is as follows: Bacillus highlandi is inoculated on the sterilized slant medium I through an inoculation loop, and cultured at 30° C. for 1 day.

[0037] In step (1), the step of activating the hal...

Embodiment 2

[0065] Embodiment 2: the preparation method of high-efficiency composting bacterial agent

[0066] Step 1. Preparation of mixed microbial bacterial liquid

[0067] (1) Slope culture of strains

[0068] Bacillus highlandi, Bacillus halotolerant, Bacillus methylotrophic, Nitrogenobacter phaeotrophus, Bacillus cereus, Candida utilis, and Bacillus amyloliquefaciens in the seed preservation tube were respectively activated by slant, and then inoculated in Purification operation is carried out in a petri dish to obtain Bacillus highlandi, Bacillus halotolerant, Bacillus methylotrophic, Nitrogenococcus sphaeroides, Bacillus cereus, Candida utilis, and Bacillus amyloliquefaciens pure breeds, respectively, for use.

[0069] In step (1), the step of activating Bacillus highlandi on the slant is as follows: Bacillus highlandi is inoculated on the sterilized slant medium I through an inoculation loop, and cultured at 30° C. for 2 days.

[0070] In step (1), the step of activating the ha...

Embodiment 3

[0098] Embodiment 3: the method that utilizes high-efficiency composting inoculum to carry out human excrement composting treatment

[0099]The feces were loaded into the fermentation device, mixed with straw powder to adjust the carbon-nitrogen ratio, and then the high-efficiency composting bacterial agent obtained in Example 2 of the present invention was inoculated into the composting system with 20% of the inoculum, and was periodically turned over to give oxygen.

[0100] In order to confirm the technical effect of the high-efficiency composting inoculum of the present invention in the composting of human feces, the effect obtained in Example 3 was compared with that of the control group (the control group was not inoculated with the inoculum, the same composting system was used, and the , oxygen supply) for comparison, and protein degradation effects after composting (see figure 1 ), polysaccharide degradation effect (see figure 2 ), cellulose degradation effect (see ...

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Abstract

The invention relates to a high-efficiency composting microbial inoculum based on gradient conversion of organic matters into human excrement PPLC as well as a preparation method and application thereof, and belongs to the technical field of excrement composting treatment. The problems that an existing fungicide is low in human excrement treatment speed, large in nitrogen loss and the like are solved. The method comprises the following steps: mixing a mixed microorganism bacterium solution consisting of bacillus altitudinis, bacillus halotolerant, bacillus methylotrophicus, azotobacter chroococcum, bacillus cereus, candida utilis and bacillus amyloliquefaciens with an immobilized carrier to prepare the immobilized microbial agent which can be applied to treatment of human excrement in the latrine; and the main components such as protein, soluble polysaccharide, grease, cellulose and the like are converted in a gradient manner. The efficient composting microbial inoculum obtained by the invention is used for degrading human excrement, so that the content of COD (Chemical Oxygen Demand) and ammonia nitrogen in the excrement can be reduced, the loss of nitrogen in the composting process of the human excrement is reduced, the temperature rise stage of composting can be quickly reached, the composting process of the excrement is accelerated, the composting time is shortened, and the resourceful treatment of the human excrement is realized.

Description

technical field [0001] The invention relates to a high-efficiency composting inoculant based on PPLC of organic matter cascade transformation of human excrement, a preparation method and application thereof, and belongs to the technical field of excrement composting treatment. Background technique [0002] In most rural areas of our country, dry toilets are still widely used, and the excrement inside the toilets often accumulates in the pits and cannot be treated in time, causing a series of environmental pollution problems. Therefore, the research and development of the rapid resource processing technology of human excrement becomes more and more important. [0003] Human feces are mainly feces and urine, and the main components of feces are protein, polysaccharide, cellulose, and oil. Therefore, bacterial agents can be screened for the above-mentioned organic macromolecular target substances. [0004] At present, many people in China have focused on the research and devel...

Claims

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Application Information

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IPC IPC(8): C12N11/10C12N11/084C12N1/20C12N1/16C05F17/20C05F3/04C12R1/07C12R1/065C12R1/085C12R1/72
CPCC12N11/10C12N11/084C12N1/20C12N1/16C05F17/20C05F3/04
Inventor 邢德峰蔡小雨王铭麒张达巍谢国俊刘冰峰
Owner HARBIN INST OF TECH
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