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Trulaspora delbrueckii MP023 with bacteriostatic effect and application thereof

A technology of MP023 and Torula sporogenes, applied in the field of microorganisms, can solve the problems of not being able to be practically applied, the yeast control mechanism is not very clear, etc., and achieve the effect of good market application prospects.

Active Publication Date: 2022-07-15
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, people are still not very clear about the mechanism of yeast control, which is also one of the main reasons why it cannot be used in practice.

Method used

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  • Trulaspora delbrueckii MP023 with bacteriostatic effect and application thereof
  • Trulaspora delbrueckii MP023 with bacteriostatic effect and application thereof
  • Trulaspora delbrueckii MP023 with bacteriostatic effect and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1. Isolation and screening of strains

[0035] In June 2019, samples of leaves, roots and petals of different plants were collected in Qingdao, and the lesions and necrotic parts were removed and cut into pieces for use. L yeast extract, 20g / L peptone, 20g / L glucose), then incubate at 28°C on a shaker at 180rpm for two days; take the above culture solution and dilute it with sterile water to 10 -6 times, after coating, separating and culturing on the YPD plate, a single yeast colony was obtained, and then a plurality of pure cultured yeast strains were obtained by the method of streaking on the plate.

[0036] Utilize the plate confrontation method to test the ability of yeast strains to inhibit plant pathogenic bacteria, the medium used is potato dextrose agar (PDA) medium, and the method for preparing the medium is: 200 grams of potatoes are washed and cut into pieces, and an appropriate amount of water is added. Boil for 20-30min, filter to get the potato juice, the...

Embodiment 2

[0059] Volatile gases produced by the MP023 strain were collected using a 20 ml headspace vial. Use solid phase extraction to absorb volatile gases. The solid phase extraction model is SIGMA-ALDRICH 57329-U (DVB / CAR / FDMS. Then use gas-mass spectrometer (GC-MS, TSQ series 8000) to analyze the composition of volatile gases. Chromatography The column model is Thermo TG-5SILMS, and the capillary column size is 30m×0.25mm×0.25μm). Headspace extraction was performed for 1 hour at 40°C. The injection temperature was 250°C, the carrier gas: He, and the flow rate was 1.3 ml / min. Column temperature heating program: hold at 40 °C for 2 min, then increase to 100 at 3 °C / min, hold for 0.1 min; then increase to 240 °C at 20 °C / min, hold for 5 min. Chromatograms were recorded in full scan mode, m / z 40-400 amu, and the ion source temperature was set to 230 °C. The obtained substances were automatically searched by computer and matched with the NIST Library.

[0060] The chromatogram of vo...

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Abstract

The invention discloses a strain of torulaspora delbrueckii MP023 with a bacteriostatic effect and an application of the torulaspora delbrueckii MP023. The torulaspora delbrueckii is classified and named as torulaspora delbrueckii, the torulaspora delbrueckii is preserved in the China Center for Type Culture Collection, and the preservation number of the torulaspora delbrueckii is CCTCC (China Center for Type Culture Collection) NO: M 2022264. The torulaspora delbrueckii MP023 or gas generated by the torulaspora delbrueckii MP023 can inhibit the growth of various phytopathogens such as rhizoctonia solani, strawberry root rot, alternaria alternata, botrytis cinerea and fusarium oxysporum, so that the torulaspora delbrueckii MP023 can be prepared into a biocontrol preparation for preventing and treating plant diseases caused by the phytopathogens; good market application prospects are realized.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a strain of Torucocystis der Kjeldahl MP023 with bacteriostatic effect and application thereof. Background technique [0002] At present, plant diseases are mainly controlled by chemical control, agricultural control and biological control. Chemical control is the most important control method because of its high efficiency and convenience. However, due to chemical control, long-term use of plants produces drug resistance, and pesticide residues pollute the environment. Biological control is a method of using beneficial plant microorganisms or metabolites of microorganisms to prevent and control plant diseases, including biocontrol bacteria, biocontrol fungi, biocontrol actinomycetes, biocontrol viruses, and plant resistance agents. Biological control has become a research hotspot in recent years due to its environmental friendliness. Biological control itself...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12P7/16C12P7/62C12P7/22A01N63/32A01P3/00C12R1/645
CPCC12P7/16C12P7/62C12P7/22A01N63/32
Inventor 王光远田俊婕刘子瑜薛天资梁文星
Owner QINGDAO AGRI UNIV
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