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Tomato whole genome chip and application thereof

A whole genome and reference genome technology, applied in tomato whole genome chip and its application field, can solve the problems of many typing sites, poor flexibility, and high cost of solid-phase chip typing, and achieve low typing cost and high flexibility. Effect

Pending Publication Date: 2022-07-12
河南七度农业科技有限公司 +3
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the existing solid-phase gene chips, such as the bovine 90K solid-phase chip, have the following problems in the detection process and application: first, there are many typing sites; The SNP sites around these sites cannot be typed, but the SNP sites around these sites cannot be typed. Once the solid-phase chip is designed, the sites that can be detected are fixed, cannot be added or deleted, and have poor flexibility; finally , the cost of solid-phase chip typing is high

Method used

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  • Tomato whole genome chip and application thereof
  • Tomato whole genome chip and application thereof
  • Tomato whole genome chip and application thereof

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Embodiment Construction

[0026] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, but not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0027] Attached to the following Figure 1-4 The present invention will be further described in detail with the examples, which are only used to explain the present invention, but not to limit the protection scope of the present invention.

[0028] (1) Design and preparation of tomato 1.5K liquid phase chip

[0029] The present invention utilizes the resequencing data of 325 breeding materials, firstly carries out SNP analysis, and a...

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Abstract

The invention discloses a tomato whole genome chip and application thereof, gene typing objects capable of being used for chip design are found and screened out, the gene typing objects comprise 1377 SNP loci and 80 Indel loci located on a tomato reference genome SL2.50, and tomato gene typing can be achieved through a targeted capture sequencing technology by means of a liquid phase chip; experimental results show that the chip designed by the invention can be used for tomato genetic diversity analysis, variety identification, genetic relationship identification, whole genome association analysis and genome selective breeding.

Description

technical field [0001] The invention belongs to the technical field of whole genome gene chips, in particular to a tomato whole genome chip and its application. Background technique [0002] Molecular Marker Technology is an important tool in molecular breeding. Traditional molecular markers, such as Restriction Fragment Length Polymorphism (RFLP) and Simple Sequence Repeat (SSR), play an important role in the field of genetics and breeding. However, there are also certain limitations, such as the small number of genomes distributed, the cumbersome operation process and low throughput, which make it impossible to meet the needs of large-scale commercial breeding applications. Single Nucleotide Polymorphism (Single Nucleotide Polymorphism, SNP) refers to the variation of a single nucleotide in the genome, including single base pair conversion, transversion, insertion or deletion. As a more widely distributed genetic marker in the genome, SNP has the characteristics of high ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6874C12N15/11
CPCC12Q1/6895C12Q1/6874C12Q2600/156C12Q2535/122
Inventor 李纪锁贾芝琪康东木贾明朝张砚迪李营
Owner 河南七度农业科技有限公司
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