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Genome selection method for laying number of white feather broilers

A technology for white-feathered broiler chickens and egg production, which is applied in the fields of genetic breeding and molecular biology, can solve the problems of time-consuming and resource-consuming, impractical poultry GS breeding practices, etc., to save costs, alleviate the dependence of provenance on foreign breeds, and improve progress slow effect

Pending Publication Date: 2021-10-26
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Bayes method can weight different effect SNPs, but it is not practical for poultry GS breeding practice because it is time-consuming and resource-intensive

Method used

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  • Genome selection method for laying number of white feather broilers
  • Genome selection method for laying number of white feather broilers
  • Genome selection method for laying number of white feather broilers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0125] Example 2 Effects of 5 SNP markers on egg production traits

[0126] The effects of five significant SNP loci on egg production traits are shown in Table 5.

[0127] Table 5 Effects of 5 significant SNPs on egg production traits (least square mean ± standard deviation)

[0128]

[0129] Note: Different shoulder letters indicate significant difference (P<0.05).

Embodiment 3

[0130] Example 3 Evaluation of the effect of genomic selection on egg production number of white-feathered broiler chickens by integrating significant SNP markers

[0131] 1. Use white-feathered broiler chickens to test the population, 2838, two generations. The phenotype records the number of individual eggs per day from 174 to 354 days. The total number of eggs laid at the age of 174-354 days for each individual was counted.

[0132] In this example, the target trait of genome selection is the number of eggs laid. A 55K SNP chip was used for genome-wide SNP typing and 5 most significant SNP typing, and a kinship matrix was constructed respectively. The two matrices were given different weights, and combined with the pedigree-based kinship matrix to form an H matrix. Then, the 5-fold cross-validation method was used to randomly divide the two generation groups, that is, 2838 chickens were randomly divided into 5 uniform groups, with 567 chickens in each group. Among the 5 ...

Embodiment 4

[0165] Embodiment 4 Utilizes the breeding method of the genomic selection of egg production number of white-feathered broiler chickens by integrating significant SNP markers

[0166] 1. Establishment of reference population, determination of phenotypic traits and genotype

[0167] Establish an independent reference group for each strain, and the source of the reference group is required to cover all existing families of the strain. When the reference flock is kept close to 52 weeks of age, a flock of 1000-1500 chickens is formed as the reference flock. The reference group has clear phenotype records (see Example 3 for methods), pedigree records, blood samples are collected, DNA is extracted, and sent to chicken genome-wide SNP chips for testing. Determine the genotypes of 30,000 to 50,000 SNP sites distributed evenly across the genome. For specific procedures, please refer to ZL201780023241.X (a chicken genome-wide SNP chip and its application). Determine the results of abo...

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Abstract

The invention provides a genome selection method for the laying number of white feather broilers, which is used for carrying out GEBV estimation on the laying number of the white feather broilers by utilizing a one-step whole genome breeding value (GEBV) estimation method integrating a remarkable SNP marker effect. The invention further provides an SNP marker remarkably related to the laying number of the white feather broilers and application of the SNP marker. The SNP marker comprises a marker rs317316870, a marker rs313925951, a marker rs14786427, a marker rs14756086 and a marker rs316455929. Compared with a conventional one-step GEBV estimation result, the breeding method of the white feather broiler strain with the low laying number provided by the invention has the advantages that: the selection accuracy can be improved by 8.1%; the new strain breeding time can be shortened; the breeding cost is saved; the breeding efficiency is improved; and the breeding process of the white feather broiler autonomous strain in China is accelerated.

Description

technical field [0001] The invention relates to the fields of genetic breeding and molecular biology, in particular to a genome selection method for laying number of white-feathered broiler chickens. Background technique [0002] my country ranks first in the world in the number of broiler chickens slaughtered, with an annual slaughter of more than 10 billion. In recent years, with the change of my country's meat consumption structure, the consumption of chicken has shown a trend of increasing year by year, and has now become the second largest consumption of meat in my country. Although the chicken output is large enough, the excellent breeds independently bred in my country still lag behind foreign countries, and the white-feathered broiler grandparents are almost entirely dependent on imports, so domestic broiler breeding is still facing enormous pressure. my country's chicken meat mainly comes from the imported white-feather broilers with fast growth characteristics and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11G16B20/00
CPCC12Q1/6888G16B20/00C12Q2600/124C12Q2600/156C12Q2600/172Y02P60/87
Inventor 赵桂苹李庆贺丁纪强朱丹郑麦青刘冉冉营凡王杰李森
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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