Saccharide composition, glycosyl composition and cryopreservation liquid

Abstract

The invention discloses a saccharide composition. The saccharide composition comprises at least one of trehalose, cane sugar, melibiose and mannan. The invention further provides a glycosyl composition, a cryopreservation solution and application of the cryopreservation solution. According to the freezing medium provided by the invention, low-dose DMSO and dextran 40 are selected, a combination of trehalose, cane sugar, melibiose and mannan is used for replacing fetal calf serum (FBS) to be used as a basic formula of the freezing medium for composition, and inhibitors of vitamin C, resveratrol, taurine, L-arginine and Caspase9 are added, so that the freezing medium is prepared. And the cells subjected to low-temperature cryopreservation can still keep good cell activity after being recovered according to a specific program. The pollution of pathogenic bacteria can be reduced, and the safety is higher; meanwhile, the doping of heterologous protein is avoided, the use amount of DMSO is reduced, and the clinical use risk can be reduced.

Description

technical field [0001] The present application relates to the technical field of biomedicine, and in particular to a carbohydrate composition, a carbohydrate-based composition and a freezing solution. Background technique [0002] Cryopreservation is a widely used method for long-term preservation of biological samples such as cells. Usually cryopreservation is to use a cryoprotectant and a special method to cool the living biological samples to a low temperature (-196°C), and then store them for a long time. When needed, the biological sample is heated and thawed according to a suitable method, and a living biological sample can still be obtained. As immunotherapy and gene therapy have become the research hotspots of many researchers at this stage, the cryopreservation of related immune cells and stem cells is particularly important. [0003] The main components of the existing cryopreservation agent are composed of DMSO, dextran, fetal bovine serum, etc., which have two ...

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Problems solved by technology

[0003] The main components of the existing cryoprotectant are composed of DMSO, dextran, fetal bovine serum, etc., which have two obvious shortcomings. One is that most of the cryopreservation solutions use fetal bovine serum FBS as the cryoprotectant The main raw material of the cryoprotectant is that the cells have heterologous proteins derived from animal serum, which makes it difficult to avoid contamination by pathogenic bacteria

Second, although the use of DMSO as a cryoprotectant is the gold standard for cell cryopreservation, high-concentration DMSO is not suitable for cryopreservation of clinically used cells because it may be toxic to the body and cause a wide range of adverse events

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