Immobilized alkaline protease and preparation method thereof

Abstract

The invention discloses immobilized alkaline protease and a preparation method thereof, and relates to the technical field of biological catalysis. The method specifically comprises the following steps: calcining cuticles and / or prismatic layers in shells as raw materials to obtain shell powder; the shell powder is utilized to adsorb alkaline protease, and then the alkaline protease is wrapped in gelatin / chitosan composite microcapsules to obtain the immobilized alkaline protease. The preparation method disclosed by the invention is simple and environment-friendly, the obtained immobilized alkaline protease product has the characteristics of high activity, high stability and high load and can be repeatedly utilized for multiple times, the preparation process is green, simple and convenient, waste shell resources can be reasonably utilized, and ecological environment pollution is reduced.

Description

technical field [0001] The invention relates to the technical field of biocatalysis, in particular to an immobilized alkaline protease and a preparation method thereof. Background technique [0002] As a biological catalyst, protease has many advantages, such as high catalytic efficiency, strong reaction specificity, few harmful by-products, and mild reaction conditions. However, as a protein, the structure of protease is very sensitive to the external environment, and its volatile inactivation; even under the most suitable conditions, with the prolongation of the reaction time, the enzyme activity is also declining; and the protease is generally water-soluble However, recycling is difficult and pollution increases, and these disadvantages limit its wider application. [0003] The immobilized enzyme is in a locked state in a certain space, which helps to improve the stability of the enzyme and improve the efficiency of the enzyme; the immobilized enzyme after the reaction i...

AI Technical Summary

Problems solved by technology

However, protease, as a kind of protein, is very sensitive to the external environment in its structure, and is inactivated due to variability; even under the most suitable conditions, with the prolongation of the reaction time, the enzyme activity is also declining; and proteases are generally water-soluble However, it is difficult to recycle and increase pollution, these shortcomings limit its wider application

[0003] The immobilized enzyme is in a closed state in a certain space, which helps to improve the stability of the enzyme and improve the use efficiency of the enzyme; the immobilized enzyme after the reaction is easy to separate from the substrate and product, and can be used repeatedly; but in the immobilization process The active conformation of the enzyme protein often changes, which reduces the activity of the immobilized enzyme. How to improve the activity of the immobilized enzyme remains to be studied by scholars at home and abroad.

Images