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Method for inducing bougainvillea speetabilis polyploidy in vitro

A bougainvillea and polyploid technology, applied in the field of in vitro induction of bougainvillea polyploidy, can solve the problems of limited hybrid varieties, low pollen activity, lack of orientation, etc., and achieves increased breeding routes, extended viewing period, and rapid growth. Effect

Pending Publication Date: 2022-06-28
TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Because bougainvillea generally has problems such as low pollen activity and self-hybridization incompatibility, the development of its large-scale hybrid breeding is restricted, and hybrid varieties are very limited; most of the results obtained by mutation breeding are single traits such as flower color and leaf color. Variation, low mutagenesis rate, less beneficial variation, and lack of orientation; ploidy breeding can double the chromosomes of plants and change the characteristics of varieties at the genetic level. Polyploid plants often show more spots, bright colors, and long flowering periods , leaf hypertrophy, enhanced resistance, etc.

Method used

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  • Method for inducing bougainvillea speetabilis polyploidy in vitro
  • Method for inducing bougainvillea speetabilis polyploidy in vitro

Examples

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Effect test

Embodiment 1

[0026] Example 1: A method for inducing bougainvillea polyploidy in vitro, selecting the diploid bougainvillea hairy species as the female parent and the diploid light-leaf species as the male parent for hybridization, and collecting mature triangles after 30 days of pollination. After plum hybrid seeds, mature bougainvillea hybrid seeds are collected. The selected seeds are first washed with detergent, soaked and disinfected with Xinjieerfang, rinsed with high-temperature sterilized sterile water, and then spread on a paper towel at certain intervals. In a culture box fully moistened with sterile water, culture in the dark in an artificial climate box with a temperature of 22-24°C to induce seed germination;

[0027] After 15 days of induction and culture, root hairs germinate and the hypocotyls grow to 5-6cm. When the two cotyledons are fully expanded, take them out, cut off the root hairs with a sterile blade, place the hypocotyls in a beaker, wash with detergent, and rinse ...

Embodiment 2

[0034]Example 2: A method for inducing bougainvillea polyploid in vitro, selecting the diploid bougainvillea light-leaf species as the female parent and the diploid light-leaf species as the male parent for hybridization, and collecting mature triangles after 25 days of pollination. After plum hybrid seeds, mature bougainvillea hybrid seeds are collected. The selected seeds are first washed with detergent, soaked and disinfected with Xinjieerfang, rinsed with high-temperature sterilized sterile water, and then spread on a paper towel at certain intervals. In a culture box fully moistened with sterile water, culture in the dark in an artificial climate box with a temperature of 22-24°C to induce seed germination;

[0035] After 10 days of induction and culture, root hairs germinate and the hypocotyl grows to 5-6 cm. When the two cotyledons are fully unfolded, take them out, cut off the root hairs with a sterile blade, place the hypocotyl in a beaker, wash with detergent, and rin...

Embodiment 3

[0042] Example 3: A method for inducing bougainvillea polyploidy in vitro, selecting the diploid bougainvillea hairy species as the female parent and the diploid light-leaf species as the male parent for hybridization, and collecting mature triangles after 28 days of pollination. After plum hybrid seeds, mature bougainvillea hybrid seeds are collected. The selected seeds are first washed with detergent, soaked and disinfected with Xinjieerfang, rinsed with high-temperature sterilized sterile water, and then spread on a paper towel at certain intervals. In a culture box fully moistened with sterile water, culture in the dark in an artificial climate box with a temperature of 22-24°C to induce seed germination;

[0043] After 12 days of induction and culture, root hairs germinate and the hypocotyl grows to 5-6 cm. When the two cotyledons are fully unfolded, take them out, cut off the root hair with a sterile blade, place the hypocotyl in a beaker, wash with detergent, and rinse w...

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Abstract

The invention provides a method for in-vitro induction of bougainvillea speetabilis polyploidy, which comprises the following steps: hybridizing a diploid bougainvillea speetabilis var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra var. Glabra and inoculating the pre-cultured hypocotyl into a differential culture medium, performing polyploidy induction culture for 30 days, and sequentially performing multiplication culture and rooting culture on survived differentiated buds in a multiplication culture medium and a rooting culture medium. Colchicine containing dimethyl sulfoxide is used for carrying out doubling induction on a bougainvillea speetabilis sterile material, so that bougainvillea speetabilis chromosomes are doubled, a polyploidy plant is obtained, and breeding ways of bougainvillea speetabilis are increased. The plant polyploid not only has the advantages of strong growth vigor, rapid growth, prolonged ornamental period, strong comprehensive resistance and the like, but also can improve the plant fertility, and can provide new germplasm for large-scale bougainvillea speetabilis crossbreeding.

Description

technical field [0001] The invention relates to the induction of polyploidy, in particular to a method for inducing bougainvillea polyploidy in vitro. Background technique [0002] Bougainvillea, also known as bougainvillea, bougainvillea, bougainvillea, etc., is a general term for ornamental plants of the genus Bougainvillea in the Nyctaginacea family. It is native to Brazil and Peru in South America, and was introduced by France in 1768. Botanist Jeanne Baré first discovered it in Rio de Janeiro, Brazil, and then named the plant after the French seafaring explorer (Louis-antoine de Bougainville). With its introduction and domestication, it is now found in major tropical and subtropical regions of the world. Bougainvillea has large bracts, bright colors like flowers, and lasts for more than half a year, and has high ornamental value; its plants are evergreen all year round, with developed root system, drought resistance, barrenness, and good climate adaptability; Bougainvi...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H4/00
CPCA01H1/02A01H4/002A01H4/008
Inventor 冷青云陆锦萍牛俊海徐世松
Owner TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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