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Reagent and method for detecting whether added sample amount is abnormal or not

A technology for detecting the amount of reagents and samples, which is used in the field of reagents to detect whether the amount of added samples is abnormal, and can solve problems such as small fluctuations in content

Pending Publication Date: 2022-06-24
SINOCARE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] All human blood samples contain human albumin, and its content fluctuates in a small range (for example, the content in normal human serum / plasma is usually 35g / L-55g / L, and the more likely content range is 40g / L-50g / L L, if it exceeds this range, there will be very obvious clinical indications, but there is currently no method to use albumin as a detection marker to judge whether the sample loading is abnormal

Method used

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  • Reagent and method for detecting whether added sample amount is abnormal or not
  • Reagent and method for detecting whether added sample amount is abnormal or not
  • Reagent and method for detecting whether added sample amount is abnormal or not

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] ① Target items to be tested: C-reactive protein;

[0078] ②Experimental materials: The samples to be tested are fresh human serum samples;

[0079] ③Reagent1: 4-hydroxyethylpiperazine ethanesulfonic acid buffer 50mM~500mM; NaCl 150mM~500mM;

[0080] ④Reagent2: 4-hydroxyethylpiperazine ethanesulfonic acid buffer 50mM~500mM; albumin blue 0.05%~2%; C-reactive protein antibody coating latex 0.1%~0.5%;

[0081] ⑤Albumin concentration determination reagent threshold: 3.94~6.56;

[0082] ⑥ Detection process: The reagent / sample needle transfers 150μl of Reagent 1 to the reaction chamber, and then the reagent / sample needle transfers 150μl of Reagent 2 to the aforementioned reaction chamber and mixes it evenly, activate the fluorescent light source to read the fluorescence intensity F0, and activate the UV light at the same time The light source reads A0. Reagent / sample needle Transfer 3 μl of the serum sample to be tested into the aforementioned reaction cup / chamber and mix e...

Embodiment 2

[0090] ①The target item to be measured: total bilirubin;

[0091] ②Experimental materials: The samples to be tested are fresh human serum samples;

[0092] ③Reagent1: Morpholine ethanesulfonic acid buffer 50mM~250mM; KCl 150mM~500mM; Tween 20 0.05%~0.2% (w / v);

[0093] ④Reagent2: Phosphate buffer 50mM~500mM; Albumin blue 0.1%~4%; Sodium metavanadate 1mM~10mM;

[0094] ⑤Albumin concentration determination reagent threshold: 13.22~21.93

[0095] ⑥ Detection process: The reagent / sample needle transfers 240μl of Reagent 1 to the reaction chamber, and then the reagent / sample needle transfers 60μl of Reagent 2 to the aforementioned reaction chamber and mixes it evenly, activate the fluorescent light source to read the fluorescence intensity Fo, and activate the UV light at the same time The light source reads A0. Reagent / sample needle Transfer 10 μl of the serum sample to be tested into the aforementioned reaction cup / chamber and mix evenly, incubate for 5 to 30 seconds, and acti...

Embodiment 3

[0103] ① Target project to be tested: cholinesterase;

[0104] ②Experimental materials: The samples to be tested are fresh human serum samples;

[0105] ③Reagent1: pyrophosphate buffer 50mM~250mM; 6-cyanoferrate 0.5mM~5mM;

[0106] ④Reagent2: pyrophosphate buffer 50mM~500mM; albumin blue 0.1%~4%; butyrylthiocholine 5mM~25mM;

[0107] ⑤Albumin concentration determination reagent threshold: 6.21~11.40;

[0108] ⑥ Detection process: The reagent / sample needle transfers 240μl of Reagent 1 to the reaction chamber, and then the reagent / sample needle transfers 60μl of Reagent 2 to the aforementioned reaction chamber and mixes it evenly, activate the fluorescent light source to read the fluorescence intensity Fo, and activate the UV light at the same time The light source reads A0. Reagent / sample needle Transfer 5 μl of the serum sample to be tested into the aforementioned reaction cup / chamber and mix evenly, incubate for 5 to 30 seconds, and activate the ultraviolet light source to...

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Abstract

The invention relates to the technical field of blood detection, in particular to a reagent and method for detecting whether the added sample amount is abnormal or not. According to the method, human serum albumin in a to-be-detected sample is detected based on a fluorescence reaction of albumin and albumin blue, a reasonable emission fluorescence acceptance range is set according to the sample adding amounts of different items, and if the acceptance range is exceeded, the sample adding amount in the testing process is judged to be abnormal; and prompting that the test result is invalid and needs to be retested or prompting that the albumin concentration needs to be rechecked. Experiments show that the method provided by the invention is accurate and reliable, and can effectively reduce the risk of misinformation of results during detection.

Description

technical field [0001] The invention relates to the technical field of blood detection, in particular to a reagent and a method for detecting whether the amount of added sample is abnormal. Background technique [0002] In the field of biochemical detection, immunoassay and other fields involving blood or urine detection in in-vitro diagnostics, abnormal sample volume (less than or higher than the target sample size), which will lead to reporting erroneous test results, resulting in clinical misdiagnosis, which may ultimately have serious consequences for patients. [0003] In the fields of biochemical testing and immunoassays, there is no systematic solution for adding abnormal sample size, and it mostly relies on the experience of testing doctors or clinicians to make judgments. [0004] Human blood samples all contain human serum albumin, and its content fluctuates within a small range (for example, the content in normal human serum / plasma is usually 35g / L~55g / L, and the...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/33G01N21/64G01N21/75G01N21/78G01N33/68
CPCG01N21/31G01N21/33G01N21/6428G01N21/75G01N21/78G01N33/68
Inventor 李宗祥汪长海罗继全周倩戴斌
Owner SINOCARE
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