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Screening and identifying method of phosphorus-solubilizing strain and application of phosphorus-solubilizing strain in biological fertilizer

An identification method and biofertilizer technology, applied in the field of bioengineering, can solve problems such as environmental pollution and soil phosphorus deficiency, and achieve the effect of improving the current situation of soil phosphorus deficiency

Pending Publication Date: 2022-06-07
WUHAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the insoluble inorganic phosphorus in the soil can be activated and fully utilized, the current situation of soil phosphorus deficiency can be solved. Although the chemical fertilizers currently used can solve this problem well, they will cause environmental pollution.

Method used

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  • Screening and identifying method of phosphorus-solubilizing strain and application of phosphorus-solubilizing strain in biological fertilizer
  • Screening and identifying method of phosphorus-solubilizing strain and application of phosphorus-solubilizing strain in biological fertilizer
  • Screening and identifying method of phosphorus-solubilizing strain and application of phosphorus-solubilizing strain in biological fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for screening and identifying phosphorus-dissolving strains, comprising the steps of:

[0023] 1. Soil collection

[0024] The soil 5-10 cm below the surface layer of the open space behind the Wuhan University of Science and Technology Library was collected, put into a sterilized kraft paper bag, and stored in the upper layer of the refrigerator at 4°C.

[0025] 2. Isolation and purification of phosphorus-dissolving strains:

[0026] Weigh 5g soil samples under sterile conditions and add them to 100mL NBRIP liquid medium (NBRIP liquid medium (g / L): glucose 10.0, Ca 3 (PO 4 ) 2 25.0, MgSO 4 ·7H 2 O 0.25, MgCl 2 ·7H 2 O 5.0, KCl 0.2, (NH 4 ) 2 SO 4 0.1, add deionized water to 1L, pH 6.8~7.0), after 30 ℃ constant temperature shaking culture for 3d, take the bacterial solution and dilute it to 10 -3 -10 -7 Five concentration gradients, each gradient was repeated 3 times, inverted in a 30°C incubator, and the colony growth on the plate was observed aft...

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Abstract

The screening and identifying method comprises the following steps: (1) collecting soil; (2) separating and purifying the phosphorus-solubilizing strain; (3) identifying the strain; and (4) determining the phosphorus-solubilizing capability. According to the method, the phosphorus-solubilizing strains can be well screened, the phosphorus-solubilizing strains with good quality can be obtained and applied to the bio-fertilizer, and the bio-fertilizer can well improve the current situation of phosphorus deficiency of soil and has the advantages of being green, efficient and environmentally friendly.

Description

technical field [0001] The invention belongs to bioengineering technology, in particular to a method for screening and identifying phosphorus-dissolving strains and its application in biological fertilizers. Background technique [0002] Phosphorus is an essential element for plant growth and plays a vital role in plant growth and development. A large area of ​​land in my country is deficient in phosphorus. Most of the phosphorus in the soil exists in the form of insoluble phosphorus. In the process of agricultural planting, phosphorus is generally supplemented by the application of phosphorus fertilizer. If the insoluble inorganic phosphorus in the soil can be activated and fully utilized, the current situation of soil phosphorus deficiency can be solved. Although the chemical fertilizers currently used can solve this problem well, it will bring environmental pollution problems. Through the method of microbial activation, the insoluble phosphorus in the soil is released, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12N1/02C12Q1/689C05G3/80C12R1/01
CPCC12Q1/04C12N1/02C12Q1/689C05F1/00C05G3/80G01N2333/195C05F5/00C05F11/00C05F11/08Y02W30/40
Inventor 高威
Owner WUHAN UNIV OF SCI & TECH
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