Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting diversity influence of pinkflower clover on constructed wetland substrate microorganisms and application of method

A technology of artificial wetlands and detection methods, which is applied in the measurement/inspection of microorganisms, biochemical equipment and methods, biostatistics, etc., and can solve problems such as high construction costs, small hydraulic loads, and weak processing capabilities

Pending Publication Date: 2022-06-03
WENZHOU MEDICAL UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Constructed wetlands with surface flow have the disadvantages of large area, small hydraulic load, weak treatment capacity, and easy breeding of mosquitoes in summer.
The water purification ability of the vertical subsurface flow constructed wetland is the strongest among the three types of constructed wetlands, but the hydraulic flow of the sewage is short, the denitrification effect is weak, and the construction cost is high and the operation management is complicated

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting diversity influence of pinkflower clover on constructed wetland substrate microorganisms and application of method
  • Method for detecting diversity influence of pinkflower clover on constructed wetland substrate microorganisms and application of method
  • Method for detecting diversity influence of pinkflower clover on constructed wetland substrate microorganisms and application of method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1 Materials and methods

[0034] 1.1 Sample Preparation and Collection

[0035] Artificial horizontal subsurface wetlands are constructed of PVC boards and filled with gravel (diameter 4-6mm) to form a filler matrix, the size of which is as follows figure 1 shown. A perforated tube is installed on the side of the device to facilitate sampling, and the collected sample is a gravel surface matrix. The planting density of windmill grass is about 25 plants per square meter, and the water flow direction is from left to right. Four areas 1, 3, 7, and 9 were selected as the sampling sites for this experiment. At the same time, groups were formed according to whether the windmill grass was planted or not. The unplanted windmill grass group was named group B: B1, B3, B7, and B9. The group of planting windmill grass was named as C group: C1, C3, C7, C9.

[0036] 1.2 Detection process

[0037] Take 1 g of sample and use a DNA extraction kit (OMEGA Soil DNA Kit) according t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention provides a method for detecting the diversity influence of pinkflower grass on constructed wetland matrix microorganisms and application. The method comprises the following steps: (1) preparing and collecting a sample; (2) extracting DNA of a sample, detecting the concentration and purity of the DNA, and then detecting the integrity of the DNA sample; (3) carrying out PCR (Polymerase Chain Reaction) amplification; (4) performing high-throughput sequencing; (5) performing quality control, filtering, splicing and chimera removal; performing taxonomic analysis on the OTU representative sequence, and calculating alpha diversity according to the OTU data to obtain a correlation index; according to the invention, by comparing the community composition difference of a gravel surface matrix biological membrane under the condition of planting or not planting pinkgrass, amplifying and sequencing the bacterium 16S rDNA gene by using a high-throughput sequencing technology, and comparing the diversity of microorganisms in different constructed wetland environments, the flora structure and dominant bacteria of the microorganisms can be researched, and the application of the bacteria 16S rDNA gene to the artificial wetland environment can be realized. And help is provided for finally designing a more scientific constructed wetland scheme.

Description

technical field [0001] The invention relates to a method for detecting the influence of plants on the microbes of a constructed wetland matrix, in particular to a method and application for detecting the influence of the windmill grass on the diversity of the microbes of the constructed wetland matrix. Background technique [0002] Wetlands are a transitional zone, between terrestrial and aquatic ecosystems. Constructed wetlands, on the other hand, refer to an engineered wetland system that can be adjusted by man-made means. It is widely used in the field of water purification because of its ecological adjustment, reliable effect, and environmental friendliness. [0003] Constructed wetlands are classified into surface flow constructed wetlands, vertical subsurface flow constructed wetlands and horizontal subsurface flow constructed wetlands according to different hydraulic modes in operation. Surface flow constructed wetlands have disadvantages such as large area, small hy...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6869C12Q1/689G16B30/00G16B40/00G16B50/00
CPCC12Q1/6869C12Q1/689G16B30/00G16B40/00G16B50/00C12Q2531/113C12Q2535/122Y02W10/10
Inventor 葛利云邓欢欢周俊威蔡贤雷
Owner WENZHOU MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com