Preparation method and application of BPN-DFO gel scaffold

Abstract

The invention relates to the technical field of biomedical materials, in particular to a preparation method and application of a BPN-DFO gel scaffold. Black phosphorus nanosheets (BPN) and deferoxamine (DFO) serve as bioactive components for promoting blood vessel and bone regeneration and are used for repairing ischemic bone defects, BPN can release controllable phosphorus, DFO serves as an iron chelating agent, angiogenesis can be promoted by activating vascular endothelial growth factors (VEGF) and other key angiogenesis promoting genes, and the effect of repairing ischemic bone defects is achieved. The gel scaffold prepared by the invention has good swelling, degradation and release rates, and has good biocompatibility. The BPN-DFO (black phosphorus nanosheet and deferoxamine) gel scaffold shows that the expression of mRNA (messenger ribonucleic acid) related to bone regeneration and cell proliferation is obviously up-regulated. In vivo, the BPN-DFO gel stent can significantly improve osteogenesis and neovascularization at the ischemic bone defect part.

Description

technical field [0002] The invention relates to the technical field of biomedical materials, in particular to a preparation method and application of a BPN-DFO gel stent. Background technique [0003] It is widely believed that delayed union or nonunion during bone healing is due to insufficient blood supply. Although bone tissue has good regeneration ability, most of ischemic bone defects cannot be fully recovered due to insufficient blood supply, which is one of the main reasons for nonunion. When nonunions are present, patients may experience bone loss, bone deformities, and persistent infection. To date, a wide range of clinical strategies for the treatment of nonunion include small defect autologous bone grafting, bone transport, and induced membrane techniques. But the above scheme is prone to a series of unavoidable complications. [0004] Recently, hydrogel scaffolds have been recognized as an effective material with a variety of medical values, including simple c...

AI Technical Summary

Problems solved by technology

In the ischemic state, these scaffolds may induce fibrous tissue formation in the bone defect and fail to treat the bone defect

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